Comparison of Clostridium difficile minimum inhibitory concentrations obtained using agar dilution vs broth microdilution methods. (April 2017)

• Record Type: Journal Article
• Authors: Hastey, Christine J.; Dale, Suzanne E.; Nary, Julia; Citron, Diane; Law, Jennifer H.; Roe-Carpenter, Darcie E.; Chesnel, Laurent
• Is Part Of: Anaerobe. Volume 44(2017)
• Page Start: 73
• Page End: 77
• Publication Date: 2017-04
• Subjects: C. difficile -- Susceptibility testing -- MIC determination; Anaerobic infections -- Periodicals; Anaerobic bacteria -- Periodicals; Bacterial diseases -- Periodicals; Computer network resources; Anaerobic protozoa -- Periodicals; 579.3
• DOI: 10.1016/j.anaerobe.2017.02.006
• Languages: English
• ISSNs: 1075-9964

Abstract

Abstract: Due to increasing antibiotic resistance among anaerobic bacteria, routine antimicrobial susceptibility testing is recommended by the Clinical and Laboratory Standards Institute (CLSI). This study compared the minimum inhibitory concentrations (MICs) from 920 Clostridium difficile isolates tested against seven antimicrobial agents using the two current CLSI reference methodologies, agar dilution method, vs broth microdilution method. A subset of isolate testing was performed independently by two laboratories to evaluate reproducibility. A negative bias was noted for MICs generated from broth microdilution compared to agar dilution and the reproducibility was variable and drug dependent. Therefore, broth microdilution is not recommended as an alternative to agar dilution for C. difficile antimicrobial susceptibility testing. Highlights: Susceptibility testing method comparison of Clostridium difficile . Agar dilution and broth microdilution MIC comparison. Inter and intra laboratory methodology comparison.