Programmed cell death‐1 expression correlates with disease severity and IL‐5 in chronic rhinosinusitis with nasal polyps. Issue 6 (6th March 2017)

• Record Type: Journal Article
• Authors: Kortekaas Krohn, I.; Bobic, S.; Dooley, J.; Lan, F.; Zhang, N.; Bachert, C.; Steelant, B.; Bullens, D. M.; Liston, A.; Ceuppens, J. L.; Seys, S. F.; Hellings, P. W.
• Is Part Of: Allergy. Volume 72:Issue 6(2017:Jun.)
• Page Start: 985
• Page End: 993
• Publication Date: 2017-03-06
• Subjects: chronic rhinosinusitis -- interleukin‐5 -- nasal polyps -- programmed cell death‐1 -- radiologic severity; Allergy -- Periodicals; 616.97
• DOI: 10.1111/all.13136
• Languages: English
• ISSNs: 0105-4538

Abstract

Abstract: Background: Programmed cell death‐1 (PD‐1) is a negative regulator of T‐cell responses. Expression of PD‐1 and its ligands PD‐L1 and PD‐L2 in chronic rhinosinusitis with nasal polyps (CRSwNP) is poorly studied. Methods: Expression of PD‐1, PD‐L1, PD‐L2, TGF‐β, IL‐5, and IL‐10 mRNA was measured by real‐time quantitative PCR on tissue homogenates of patients with CRSwNP ( n = 21) and healthy controls ( n = 21) and on primary epithelial cells. Disease severity was scored using the Lund–Mackay scores of maxillofacial computed tomography (CT) scans. Expression of PD‐1 and PD‐L1/L2 was evaluated at the cellular and tissue levels ( n = 6) by flow cytometry and immunohistochemistry. Results: Programmed cell death‐1 mRNA expression was increased in tissue homogenates from patients with CRSwNP compared with controls, irrespective of the atopy status. Importantly, expression of PD‐1 correlated with the total CT scan scores ( r = 0.5, P = 0.02). Additionally, a significant association was found between PD‐1 mRNA and expression of IL‐5 mRNA in control nasal tissue ( r = 0.95, P < 0.0001) and in CRSwNP ( r = 0.63, P = 0.002). PD‐1 was expressed on different subsets of T cells and CD11b − dendritic cells. Both PD‐1 and its ligands were expressed on primary epithelial cells from control nasal tissue and nasal polyp tissue. Conclusions: Higher PD‐1 expression was found in CRSwNP than in nasal tissue from controls. This was associated with disease severity and tissue IL‐5 expression but unrelated to the patients' atopy status.