Quantification of small GTPase glucosylation by clostridial glucosylating toxins using multiplexed MRM analysis. Issue 9 (May 2017)

• Record Type: Journal Article
• Authors: Junemann, Johannes; Lämmerhirt, Chantal M.; Polten, Felix; Just, Ingo; Gerhard, Ralf; Genth, Harald; Pich, Andreas
• Is Part Of: Proteomics. Volume 17:Issue 9(2017)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2017-05
• Subjects: Clostridium difficile -- Glucosylation -- MRM -- Small GTPases -- TcdA; Proteins -- Separation -- Periodicals; Bioinformatics -- Periodicals; Proteomics -- Periodicals; Genomes -- Periodicals; Molecular genetics -- Periodicals; 572.605
• DOI: 10.1002/pmic.201700016
• Languages: English
• ISSNs: 1615-9853

Abstract

Abstract : Large clostridial toxins mono‐O‐glucosylate small GTPases of the Rho and Ras subfamily. As a result of glucosylation, the GTPases are inhibited and thereby corresponding downstream signaling pathways are disturbed. Current methods for quantifying the extent of glucosylation include sequential [ 14 C]glucosylation, sequential [ 32 P]ADP‐ribosylation, and Western Blot detection of nonglucosylated GTPases, with neither method allowing the quantification of the extent of glucosylation of an individual GTPase. Here, we describe a novel MS‐based multiplexed MRM assay to specifically quantify the glucosylation degree of small GTPases. This targeted proteomics approach achieves a high selectivity and reproducibility, which allows determination of the in vivo substrate pattern of glucosylating toxins. As proof of principle, GTPase glucosylation was analyzed in CaCo‐2 cells treated with TcdA, and glucosylation kinetics were determined for RhoA/B, RhoC, RhoG, Ral, Rap1, Rap2, (H/K/N)Ras, and R‐Ras2.