Crystallization and preliminary X‐ray study of alanine dehydrogenase from Bacillus pseudofirmus OF4. Issue 11 (1st November 2013)

• Record Type: Journal Article
• Authors: Wen, Jinjin; Li, Zhenzhen; He, Guangzheng; Xu, Shujing; Zhao, Baohua; Zhu, Xianming; Dong, Hui; Ju, Jiansong
• Is Part Of: Acta crystallographica. Volume 69:Issue 11(2013:Nov.)
• Page Start: 1227
• Page End: 1230
• Publication Date: 2013-11-01
• Subjects: alanine dehydrogenase -- Bacillus pseudofirmus; Crystallography -- Periodicals; Crystals -- Periodicals; 548
• DOI: 10.1107/S1744309113025426
• Languages: English
• ISSNs: 1744-3091

Abstract

Abstract : Alanine dehydrogenase (OF4Ald) from the alkaliphilic Bacillus pseudofirmus OF4 was expressed and purified with a His6 tag in a form suitable for X‐ray crystallographic analysis. Crystals were grown by the hanging‐drop vapour‐diffusion method at 289 K using a solution consisting of 0.1 M Tris–HCl pH 8.0, 0.2 M LiSO4, 22%( w / v ) PEG 3350. X‐ray diffraction data were collected to 2.8 Å resolution. The crystal belonged to the triclinic space group P 1, with unit‐cell parameters a = 88.04, b = 105.59, c = 120.53 Å, α = 88.37, β = 78.77, γ = 82.65°.