Clinical applications of quantitative proteomics using targeted and untargeted data-independent acquisition techniques. (4th May 2017)

• Record Type: Journal Article
• Authors: Meyer, Jesse G.; Schilling, Birgit
• Is Part Of: Expert review of proteomics. Volume 14:Number 5(2017)
• Page Start: 419
• Page End: 429
• Publication Date: 2017-05-04
• Subjects: Data-independent acquisition -- SWATH -- mass spectrometry -- quantification -- proteomics -- clinic -- biomarker -- SRM -- PRM; Proteins -- Biotechnology -- Periodicals; Proteomics -- Periodicals; 572.6
• DOI: 10.1080/14789450.2017.1322904
• Languages: English
• ISSNs: 1478-9450

Abstract

ABSTRACT: Introduction : While selected/multiple-reaction monitoring (SRM or MRM) is considered the gold standard for quantitative protein measurement, emerging data-independent acquisition (DIA) using high-resolution scans have opened a new dimension of high-throughput, comprehensive quantitative proteomics. These newer methodologies are particularly well suited for discovery of biomarker candidates from human disease samples, and for investigating and understanding human disease pathways. Areas covered : This article reviews the current state of targeted and untargeted DIA mass spectrometry-based proteomic workflows, including SRM, parallel-reaction monitoring (PRM) and untargeted DIA (e.g., SWATH). Corresponding bioinformatics strategies, as well as application in biological and clinical studies are presented. Expert commentary : Nascent application of highly-multiplexed untargeted DIA, such as SWATH, for accurate protein quantification from clinically relevant and disease-related samples shows great potential to comprehensively investigate biomarker candidates and understand disease.