Performance assessment of a cylindrospermopsin ELISA with purified compounds and cyanobacterial extracts. Issue 2 (3rd April 2017)

• Record Type: Journal Article
• Authors: Metcalf, J. S.; Young, F. M.; Codd, G. A.
• Is Part Of: Environmental forensics. Volume 18:Issue 2(2017)
• Page Start: 147
• Page End: 152
• Publication Date: 2017-04-03
• Subjects: Cylindrospermopsin -- analysis -- false positive -- cyanobacteria -- peptides; Environmental forensics -- Periodicals; Pollution -- Measurement -- Periodicals; Environmental law -- Periodicals; Enquêtes environnementales -- Périodiques; 363.25945
• DOI: 10.1080/15275922.2017.1305011
• Languages: English
• ISSNs: 1527-5922

Abstract

ABSTRACT: Detecting low amounts of cyanobacterial toxins (cyanotoxins) in water and clinical materials is useful to protect human health and to understand cyanotoxin intoxication events. Commonly-occurring cyanobacterial compounds and extracts of cyanotoxin-producing cyanobacteria were tested by a cylindrospermopsin ELISA, in comparison with HPLC-PDA. Other than the cylindrospermopsin variants, none of the purified cyanobacterial peptides tested showed cross-reactivity in the cylindrospermopsin ELISA up to 100 µg/L, with the exception of nodularin-R. The cylindrospermopsin concentration in cylindrospermopsin-producing cyanobacteria showed good agreement between ELISA and HPLC-PDA. However, extracts of Microcystis, Planktothrix, Nodularia, and Chlorella showed ELISA positives with such non-cylindrospermopsin-producing organisms reporting concentrations greater than 1 µg/L. When cylindrospermopsins were present, the cylindrospermopsin ELISA yielded good quantification, but false positive results from non-cylindrospermopsin-containing cyanobacteria and microalgae could not be discounted.