Molecular cloning, characterization and function of a germinal center kinase MST4 gene from Litopenaeus vannamei in response to Vibrio alginolyticus challenge in TLR-TRAF6 signaling pathway. (August 2017)

• Record Type: Journal Article
• Authors: Zhao, Chang-Sheng; Huang, Di; Peng, Ting; Huang, Ming-Zhu; Xie, Chen-Ying; Chen, Jun; Kong, Jing-Rong; Xie, Ren-Chong; Liu, Yuan; Wang, Wei-Na
• Is Part Of: Developmental and comparative immunology. Volume 73(2017)
• Page Start: 206
• Page End: 219
• Publication Date: 2017-08
• Subjects: LvMST4 -- Vibrio alginolyticus challenge -- TLR-TRAF6 signal pathway -- Immunologic homeostasis -- RNAi; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2017.03.026
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: The serine/threonine protein kinase MST4 plays multiple roles in the regulation of signaling pathways that govern cellular processes including mitosis, migration, homeostasis, polarity, proliferation, differentiation and apoptosis. Here we report the identification and characterization of the full-length sequence of Lv MST4 from the shrimp L. vannamei, and investigations into its role in the shrimp's immune response to infection by the pathogenic bacterium Vibrio alginolyticus . Subcellular localization assays demonstrated the enzyme's presence in the shrimp's cytoplasm, and tissue-specific expression analysis revealed that it is expressed ubiquitously but at different levels in different tissues. Infection with V. alginolyticus increased Lv MST4 expression and induced a rapid response via the TLR-TRAF6 signaling pathway, causing a decline in the total hemocyte count (THC) and an increase in respiratory burst (RB) activity. In non-infected shrimp, RNAi silencing of Lv MST4 with dsRNA had no significant effect on THC but seemed to activate the TRAF6-MKK6-p38 pathway and reduced RB activity. In shrimp challenged with V. alginolyticus, Lv MST4 silencing reduced bacterial clearance and increased the initial upregulation of Lv TRAF6 while reducing the expression of Lv MKK6 and Lv p38. Lv MST4 silencing also slightly reduced the THC but caused pronounced increases in RB activity and cumulative mortality. These findings suggest that Lv MST4 contributes to antimicrobial responses via the TLR-TRAF6 signal pathway, and helps maintain immunological homeostasis in L. vannamei . Highlights: A crustacean a germinal center kinase MST4 gene, was identified in Litopenaeus vannamei. Lv MST4 is distributed in cytoplasm. The transcription of Lv MST4 after V. alginolyticus challenge indicated that it is involved in pathogen infection. Lv MST4 might take part in antimicrobic immunity through TLR-TRAF6 signal pathway and keep immunologic homeostasis in shrimp.