Amphiphilic/fluorous random copolymers as a new class of non-cytotoxic polymeric materials for protein conjugation. Issue 2 (7th November 2014)

• Record Type: Journal Article
• Authors: Koda, Yuta; Terashima, Takaya; Sawamoto, Mitsuo; Maynard, Heather D.
• Is Part Of: Polymer chemistry. Volume 6:Issue 2(2015)
• Page Start: 240
• Page End: 247
• Publication Date: 2014-11-07
• Subjects: Polymers -- Periodicals; Macromolecules -- Periodicals; Polymerization -- Periodicals; 547.705
• DOI: 10.1039/c4py01346h
• Languages: English
• ISSNs: 1759-9954

Abstract

Abstract : Amphiphilic/fluorous random copolymers bearing poly(ethylene glycol) chains and perfluorinated alkane pendants were developed as novel non-cytotoxic polymeric materials for protein conjugation. Abstract : Herein, amphiphilic/fluorous random copolymers bearing poly(ethylene glycol) (PEG) chains and perfluorinated alkane pendants were developed as novel non-cytotoxic polymers for protein conjugation. Three kinds of random copolymers with different initiating terminals (carboxylic acid, pyridyl disulfide, and N -hydroxysuccinimide ester) were prepared by reversible addition–fragmentation chain transfer (RAFT) copolymerization of a PEG methyl ether methacrylate and a perfluorinated alkane methacrylate with the corresponding functional chain transfer agents. All of the polymers were soluble in water to form nanostructures with perfluorinated compartments via fluorous interaction: large aggregates from the intermolecular multi-chain association and compact unimer micelles from the intramolecular single-chain folding. Such a PEGylated and perfluorinated random copolymer was non-cytotoxic to NIH 3T3 mouse embryonic fibroblast cells and human umbilical vein endothelial cells (HUVECs). Additionally, a random copolymer with a pyridyl disulfide terminal was also successfully conjugated with a thiolated lysozyme.