Accelerated Fmoc solid-phase synthesis of peptides with aggregation-disrupting backbones. Issue 5 (5th December 2014)

• Record Type: Journal Article
• Authors: Huang, Yi-Chao; Guan, Chao-Jian; Tan, Xiang-Long; Chen, Chen-Chen; Guo, Qing-Xiang; Li, Yi-Ming
• Is Part Of: Organic & biomolecular chemistry. Volume 13:Issue 5(2015)
• Page Start: 1500
• Page End: 1506
• Publication Date: 2014-12-05
• Subjects: Chemistry, Organic -- Periodicals; Bioorganic chemistry -- Periodicals; Chemistry, Physical organic -- Periodicals; 547
• DOI: 10.1039/c4ob02260b
• Languages: English
• ISSNs: 1477-0520

Abstract

Abstract : In this work, we describe an accelerated solid-phase synthetic protocol for ordinary or difficult peptides involving air-bath heating and amide protection. Abstract : In this work, we describe an accelerated solid-phase synthetic protocol for ordinary or difficult peptides involving air-bath heating and amide protection. For the Hmsb-based backbone amide protection, an optimized acyl shift condition using 1, 4-dioxane was discovered. The efficiency and robustness of the protocol was validated in the course of preparation of classical difficult peptides and ubiquitin protein segments.