Synthesis of tumor-associated MUC1-glycopeptides and their multivalent presentation by functionalized gold colloids. Issue 1 (12th September 2014)

• Record Type: Journal Article
• Authors: Tavernaro, Isabella; Hartmann, Sebastian; Sommer, Laura; Hausmann, Heike; Rohner, Christian; Ruehl, Martin; Hoffmann-Roeder, Anja; Schlecht, Sabine
• Is Part Of: Organic & biomolecular chemistry. Volume 13:Issue 1(2015)
• Page Start: 81
• Page End: 97
• Publication Date: 2014-09-12
• Subjects: Chemistry, Organic -- Periodicals; Bioorganic chemistry -- Periodicals; Chemistry, Physical organic -- Periodicals; 547
• DOI: 10.1039/c4ob01339e
• Languages: English
• ISSNs: 1477-0520

Abstract

Abstract : The authors present the synthesis of novel MUC1-glycopeptide antigens and their multivalent presentation by gold colloids. Their biological activity was tested in a dot-blot immunoassay experiment. Abstract : The mucin MUC1 is a glycoprotein involved in fundamental biological processes, which can be found over-expressed and with a distinctly altered glycan pattern on epithelial tumor cells; thus it is a promising target structure in the quest for effective carbohydrate-based cancer vaccines and immunotherapeutics. Natural glycopeptide antigens indicate only a low immunogenicity and a T-cell independent immune response; however, this major drawback can be overcome by coupling of glycopeptide antigens multivalently to immunostimulating carrier platforms. In particular, gold nanoparticles are well suited as templates for the multivalent presentation of glycopeptide antigens, due to their remarkably high surface-to-volume ratio in combination with their high biostability. In this work the synthesis of novel MUC1-glycopeptide antigens and their coupling to gold nanoparticles of different sizes are presented. In addition, the development of a new dot-blot immunoassay to test the potential antigen–antibody binding is introduced.