Optimization of a biotechnological multiproduct batch plant design for the manufacture of four different products: A real case scenario. Issue 6 (6th March 2017)

• Record Type: Journal Article
• Authors: Sandoval, Gabriela; Espinoza, Daniel; Figueroa, Nicolas; Asenjo, Juan A.
• Is Part Of: Biotechnology and bioengineering. Volume 114:Issue 6(2017)
• Page Start: 1252
• Page End: 1263
• Publication Date: 2017-03-06
• Subjects: multi‐product batch plant -- biotechnological products -- MILP; Biotechnology -- Periodicals; Bioengineering -- Periodicals; 660.6
• DOI: 10.1002/bit.26260
• Languages: English
• ISSNs: 0006-3592

Abstract

ABSTRACT: In this work a biotechnological multiproduct batch plant that manufactures four different recombinant proteins for human application is described in some detail. This batch plant design is then optimized with regards to the size of equipment using a mixed‐integer linear programming (MILP) formulation recently developed by us in order to find a hypothetical new biotechnological batch plant based on the information of real known processes for the production of the four recombinant protein products. The real plant was divided for practical purposes into two sub‐processes or facilities: a fermentation facility and a purification facility. Knowing the specific steps conforming the downstream processing of each product, size, and time factors were computed and used as parameters to solve the aforementioned MILP reformulation. New constraints were included to permit the selection of some equipment—such as centrifuges and membrane filters—in a discrete set of sizes. For equipment that can be built according to customer needs—such as reactors—the original formulation was retained. Computational results show the ability of this optimization methodology to deal with real data giving reliable solutions for a multi‐product batch plant composed of 44 unit operations in a relatively small amount of time showing that in the case studied it is possible to save up to a 66% of the capital investment in equipment given the cost data used. Biotechnol. Bioeng. 2017;114: 1252–1263. © 2017 Wiley Periodicals, Inc. Abstract : In this work a biotechnological multiproduct batch plant that manufactures four different recombinant proteins for human application is described. The batch plant design is optimized with regards to equipment size using a mixed‐integer linear programming (MILP) formulation. The computational results show the ability of this methodology to deal with real data for a multiproduct plant composed of 44 unit operations in a small amount of time showing that in this case it is possible to save up to 66% of the capital investment.