Two novel peptides with angiotensin I converting enzyme inhibitory and antioxidative activities from Scorpaena notata muscle protein hydrolysate. (16th June 2016)

• Record Type: Journal Article
• Authors: Aissaoui, Neyssene; Abidi, Ferid; Hardouin, Julie; Abdelkafi, Zaineb; Marrakchi, Naziha; Jouenne, Thierry; Marzouki, M. Nejib
• Is Part Of: Biotechnology and applied biochemistry. Volume 64:Number 2(2017)
• Page Start: 201
• Page End: 210
• Publication Date: 2016-06-16
• Subjects: angiotensin I converting enzyme inhibitory activity -- antioxidant peptides -- hydrophilic amino acids -- protein hydrolysate -- purification and identification -- therapeutic compounds; Biotechnology -- Periodicals; Biochemical engineering -- Periodicals; Biochemistry -- Periodicals; Biochemistry -- Periodicals; Genetic Techniques -- Periodicals; Microbiological Techniques -- Periodicals; 660.6
• DOI: 10.1002/bab.1478
• Languages: English
• ISSNs: 0885-4513

Abstract

Abstract: Fish protein hydrolysate was prepared from muscle of small red scorpionfish ( Scorpaena notata ) by treatment with a protease from the fungus Penicillium digitatum . Protein hydrolysate was found to strongly inhibit the angiotensin I converting enzyme and exhibited high antioxidative activity through 1, 1‐diphenyl‐2‐picrylhydrazyl free radical scavenging assay. After ultrafiltration, peptides were isolated by a two‐step procedure: size exclusion chromatography on a Toyopearl HW‐40 followed by reversed‐phase high‐performance liquid chromatography with a high purification yield of 2.5 mg of peptide per gram of initial protein. Two major peptides were then identified by nanoscale liquid chromatography coupled to tandem mass spectrometry (nano‐LC‐MS/MS), corresponding to the following sequences: Leu‐Val‐Thr‐Gly‐Asp‐Asp‐Lys‐Thr‐Asn‐Leu‐Lys (1, 204.665 Da) and Asp‐Thr‐Gly‐Ser‐Asp‐Lys‐Lys‐Gln‐Leu (992.511 Da). These peptides, mainly composed of hydrophilic amino acids, showed high antioxidative and angiotensin I converting enzyme inhibitory activities. These data suggest that the two novel peptides isolated from the muscle hydrolysate of small red scorpionfish can be a beneficial ingredient for functional foods or pharmaceuticals against hypertension and oxidative stress.