Evaluation of the effect of Ramadan fasting on fat-soluble antioxidants and markers of oxidative stress in healthy Pakistani subjects. Issue 4 (25th February 2017)

• Record Type: Journal Article
• Authors: Khan, Abad; Iqbal, Zafar
• Is Part Of: Journal of liquid chromatography & related technologies. Volume 40:Issue 4(2017)
• Page Start: 210
• Page End: 216
• Publication Date: 2017-02-25
• Subjects: Antioxidants -- fasting -- healthy -- HPLC–UV -- samples; Liquid chromatography -- Periodicals; Chromatographic analysis -- Periodicals; Separation (Technology) -- Periodicals; Biotechnology -- Periodicals; Chromatography, Liquid -- Periodicals; 543.8405
• DOI: 10.1080/10826076.2017.1300774
• Languages: English
• ISSNs: 1082-6076

Abstract

ABSTRACT: The aim of this study is to evaluate the effect of Ramadan fasting on the fat-soluble antioxidants [all- trans -retinol (vitamin A) and α -tocopherol (vitamin E)] in healthy Pakistani subjects. Thirty (30) healthy male volunteers (aged 30–50 years) with Ramadan fasting have participated in the study. Blood sampling from these subjects was conducted 1 day before and on 15th and 28th days of Ramadan. The serum levels of vitamin A and vitamin E were measured using liquid chromatography linked with UV–visible (HPLC–UV). The comparison between the samples taken at different times was made by Minitab software applying two samples and paired t -test at ( p < 0.05) as significant. No significant changes have been found in the levels of all- trans -retinol before and during Ramadan values. The levels of α -tocopherol on 28th Ramadan have been decreased significantly when compared with its values before Ramadan ( p < 0.0001). It is concluded that there is nonsignificant change in the levels of vitamin A during the month of Ramadan while the levels of α -tocopherol have been decreased significantly on the 28th day of Ramadan. It is therefore suggested that the fasting of Ramadan has effect on the levels of α -tocopherol and food-based interventions might be necessary to modify the diet during Ramadan. GRAPHICAL ABSTRACT: