Optimizing two-phase system by experiment and simulation for high-speed counter-current chromatographic separation of 2-phenylbutyric acid enantiomer. Issue 7 (3rd May 2017)

• Record Type: Journal Article
• Authors: Zhang, Panliang; Xie, Xiaojuan; Tang, Kewen; Xu, Weifeng; Xiong, Biquan; Liu, Yu
• Is Part Of: Separation science and technology. Volume 52:Issue 7(2017)
• Page Start: 1275
• Page End: 1282
• Publication Date: 2017-05-03
• Subjects: Chiral separation -- high-speed counter-current chromatography -- hydroxyethy-β-cyclodextrin -- modelling -- 2-phenylbutyric acid; Separation (Technology) -- Periodicals; 660.284205
• DOI: 10.1080/01496395.2017.1283329
• Languages: English
• ISSNs: 0149-6395

Abstract

ABSTRACT: High-speed counter-current chromatographic preparative separation of 2-phenylbutyric acid (2-PBA) was successfully proposed. The two-phase solvent system was composed of n -hexane/butyl acetate/aqueous phase (7:3:10, v/v/v). Factors influencing the chiral separation efficiency ( e.g . types and concentrations of chiral selector (CS), pH of aqueous phase and temperature) were investigated. A mathematical model was established and verified by conducting experiments. Results showed that the model predictions were in good agreement with the experimental results. Under optimal conditions, 2-PBA was successfully separated. The monomer purity reached 99.5%, and the recovery of enantiomers was 91–93%.