A photo-degradable supramolecular hydrogel for selective delivery of microRNA into 3D-cultured cells. Issue 10 (21st February 2017)

• Record Type: Journal Article
• Authors: Zhou, Zhengquan; Yi, Qikun; Xia, Tingting; Yin, Wencui; Kadi, Adnan A.; Li, Jinbo; Zhang, Yan
• Is Part Of: Organic & biomolecular chemistry. Volume 15:Issue 10(2017)
• Page Start: 2191
• Page End: 2198
• Publication Date: 2017-02-21
• Subjects: Chemistry, Organic -- Periodicals; Bioorganic chemistry -- Periodicals; Chemistry, Physical organic -- Periodicals; 547
• DOI: 10.1039/c6ob02667b
• Languages: English
• ISSNs: 1477-0520

Abstract

Abstract : A multi-functional supramolecular hydrogel was developed for long-term 3D cell culture, targeted miRNA delivery and easy collection of encapsulated cells. Abstract : Multi-functional supramolecular hydrogels have emerged as smart biomaterials for diverse biomedical applications. Here we report a multi-functional supramolecular hydrogel formed by the conjugate of the bioactive GRGDS peptide with biaryltetrazole that is the substrate of photo-click reaction. The hydrogel was used as a biocompatible matrix to encapsulate live cells for 3D culture. The presence of the RGD epitope in the hydrogelator enhanced the interaction of the nanofiber with integrin over-expressing cells, which resulted in the selective enhancement in the miRNA delivery into the encapsulated U87 cells. The intramolecular photo-click reaction of the biaryltetrazole moiety in the hydrogelator leads to a sensitive photo-response of the hydrogel, which allowed photo-degradation of the hydrogel for release of the encapsulated live cells for further bio-assay of the intracellular species.