Niosomes based on synthetic cationic lipids for gene delivery: the influence of polar head-groups on the transfection efficiency in HEK-293, ARPE-19 and MSC-D1 cells 1. Issue 4 (21st November 2014)

• Record Type: Journal Article
• Authors: Ojeda, E.; Puras, G.; Agirre, M.; Zárate, J.; Grijalvo, S.; Pons, R.; Eritja, R.; Martinez-Navarrete, G.; Soto-Sanchez, C.; Fernández, E.; Pedraz, J. L.
• Is Part Of: Organic & biomolecular chemistry. Volume 13:Issue 4(2015)
• Page Start: 1068
• Page End: 1081
• Publication Date: 2014-11-21
• Subjects: Chemistry, Organic -- Periodicals; Bioorganic chemistry -- Periodicals; Chemistry, Physical organic -- Periodicals; 547
• DOI: 10.1039/c4ob02087a
• Languages: English
• ISSNs: 1477-0520

Abstract

Abstract : We designed niosomes based on three lipids that differed only in the polar-head group to analyze their influence on the transfection efficiency. Abstract : We designed niosomes based on three lipids that differed only in the polar-head group to analyze their influence on the transfection efficiency. These lipids were characterized by small-angle X-ray scattering before being incorporated into the niosomes which were characterized in terms of p K a, size, zeta potential, morphology and physical stability. Nioplexes were obtained upon the addition of a plasmid. Different ratios (w/w) were selected to analyze the influence of this parameter on size, charge and the ability to condense, release and protect the DNA. In vitro transfection experiments were performed in HEK-293, ARPE-19 and MSC-D1 cells. Our results show that the chemical composition of the cationic head-group clearly affects the physicochemical parameters of the niosomes and especially the transfection efficiency. Only niosomes based on cationic lipids with a dimethyl amino head group (lipid3 ) showed a transfection capacity when compared with their counterparts amino (lipid1 ) and tripeptide head-groups (lipid2 ). Regarding cell viability, we clearly observed that nioplexes based on the cationic lipid3 had a more deleterious effect than their counterparts, especially in ARPE-19 cells at 20/1 and 30/1 ratios. Similar studies could be extended to other series of cationic lipids in order to progress in the research on safe and efficient non-viral vectors for gene delivery purposes.