Cellular hydrogels based on pH-responsive chitosan-hydroxyapatite system. (15th June 2017)

• Record Type: Journal Article
• Authors: Rogina, Anamarija; Ressler, Antonia; Matić, Igor; Gallego Ferrer, Gloria; Marijanović, Inga; Ivanković, Marica; Ivanković, Hrvoje
• Is Part Of: Carbohydrate polymers. Volume 166(2017)
• Page Start: 173
• Page End: 182
• Publication Date: 2017-06-15
• Subjects: Chitosan -- Hydroxyapatite -- pH-responsive hydrogel -- Rheology -- Cell encapsulation; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2017.02.105
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: Physically crosslinked chitosan-HAp hydrogel was prepared by NaHCO3 . In situ apatite synthesis in chitosan ensured application of low NaHCO3 quantity. The gelation time of chitosan-HAp/NaHCO3 system is approximately 4 min. Cells encapsulated within chitosan-HAp/NaHCO3 show high viability. Abstract: The development of bioactive injectable system as cell carrier with minimal impact on viability of encapsulated cells represents a great challenge. In the present work, we propose a new pH-responsive chitosan-hydroxyapatite-based hydrogel with sodium bicarbonate (NaHCO3 ) as the gelling agent. The in situ synthesis of hydroxyapatite phase has resulted in stable composite suspension and final homogeneous hydrogel. The application of sodium bicarbonate has allowed non-cytotoxic fast gelation of chitosan-hydroxyapatite within 4 min, and without excess of sodium ions concentration. Rheological properties of crosslinked hydrogel have demonstrated possible behaviour as 'strong physical hydrogel'. The live dead staining has confirmed good viability and dispersion, as well as proliferation of encapsulated cells by the culture time. Presented preliminary results show good potential of chitosan-hydroxyapatite/NaHCO3 as a cell carrier, whose impact on the cell differentiation need to be confirmed by encapsulation of other cell phenotypes.