Preparation and characterization of amine functional nano-hydroxyapatite/chitosan bionanocomposite for bone tissue engineering applications. (15th May 2017)

• Record Type: Journal Article
• Authors: Atak, Besir Hakan; Buyuk, Berna; Huysal, Merve; Isik, Sevim; Senel, Mehmet; Metzger, Wolfgang; Cetin, Guven
• Is Part Of: Carbohydrate polymers. Volume 164(2017)
• Page Start: 200
• Page End: 213
• Publication Date: 2017-05-15
• Subjects: ALP alkaline phosphatase -- APTES 3-aminopropyltriethoxysilane -- BM bone marrow -- BTE bone tissue engineering -- CTS chitosan -- DMEM-LG Dulbecco's Modified Eagle's medium low glucose -- ELISA enzyme-linked immune sorbent assay -- FT-IR Fourier transform infrared spectroscopy -- hBM-MSCs human bone mesenchymal stem cells -- HMDS hexamethyldisilazane -- LDH lactate dehydrogenase -- MSC-FBS mesenchymal Stem Cell – Qualified Fetal Bovine Serum -- nHAP nano-hydroxyapatite -- PBS phosphate buffered saline -- RGD arginylglycylaspartic acid -- SEM scanning electron microscopy -- TGA thermogravimetric analysis -- WST-1 Water Soluble Tetrazolium Salt-1 -- XRD X-Ray diffractometry -- β-GP β-glycerophosphate; Scaffold -- Chitosan -- Hydroxyapatite -- Human mesenchymal stem cells -- Osteogenic differentiation; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2017.01.100
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: Amine functional nano-hydroxyapatite/chitosan bionanocomposite scaffolds for bone tissue engineering applications. Bionanocomposite and scaffolds were characterized by ATR-FTIR, SEM and TG. Cell viability and proliferation of osteoblast cells on the scaffolds were enhanced by incorporation of amine functional nHAP. Scaffolds present good properties to be evaluated as biomaterials. Abstract: In this study, three different types of scaffolds including a uniquely modified composite scaffold – namely chitosan (CTS), nano-hydroxyapatite/chitosan composite (CTS + nHAP), and amine group (NH2 ) modified nano-hydroxyapatite/chitosan composite (CTS + nHAP-NH2 ) scaffolds – were synthesized for bone tissue engineering (BTE) purposes. As results of the study, it was found that all scaffold types were biodegradable with CTS and CTS + nHAP scaffolds losing up to 15% of their initial weight, while the CTS + nHAP-NH2 scaffold showing 10% of weight loss after six weeks of lysozyme treatment. In addition, all three types of scaffolds were shown to be biocompatible, and amongst them CTS + nHAP-NH2 scaffolds supported the most cell proliferation in WST-1 assay and expressed the least and acceptable level of cytotoxicity in lactate dehydrogenase (LDH) test for human bone mesenchymal stem cells (hBM-MSCs). Finally, during osteoinductivity assessment, CTS + nHAP-NH2 nearly tripled initial alkaline phosphatase (ALP) activity when whereas both CTS and CTS + nHAP scaffolds only doubled. These results indicate that all synthesized scaffold types under investigation have certain potential to be used in bone tissue engineering approaches with CTS + nHAP-NH2 scaffold being the most promising and applicable one. In the future, we plan to intensify our studies on osteogenic differentiation on our scaffolds on a detailed molecular level and to include in vivo studies for pre-clinical purposes.