A competitive ELISA for detection of group specific antibody to bluetongue virus using anti-core antibody. (March 2017)

• Record Type: Journal Article
• Authors: Chand, Karam; Biswas, Sanchay K.; Pandey, Awadh B.; Saxena, Arpit; Tewari, Neha; Mondal, Bimalendu
• Is Part Of: Biologicals. Volume 46(2017)
• Page Start: 168
• Page End: 171
• Publication Date: 2017-03
• Subjects: C-ELISA -- Bluetongue virus -- Serogroup -- VP7 protein; Biological products -- Standards -- Periodicals; Biological Products -- Periodicals; Biological Products -- standards -- Periodicals; Produits biologiques -- Normes -- Périodiques; Biological products -- Standards; Periodicals; 615.37
• DOI: 10.1016/j.biologicals.2017.01.002
• Languages: English
• ISSNs: 1045-1056

Abstract

Abstract: Bluetongue virus (BTV) is transmitted by biting midges, which infects domestic and wild ruminants. In present study, a competitive enzyme-linked immunosorbent assay (C-ELISA) for the detection of serogroup-specific antibodies against VP7 protein of BTV has been developed. The assay measures the competition between a group specific antibody against core protein of BTV and a test serum to an optimized concentration of BTV recombinant-VP7 (r-VP7) antigen. Serum samples (n = 895) collected from small and large ruminants were used to optimize the C-ELISA. Percent inhibition (PI) values were used for estimation of the cut-off value for the C-ELISA. On receiver operator characteristic (ROC) analysis, different cut-off values along with their diagnostic sensitivity (DSn) and diagnostic specificity (DSp) were obtained. Among these, >50% PI value was accepted as cut-off at which DSn and Dsp was achieved as 97.6% and 98.0% respectively, at >95% confidence interval. Results show the present C-ELISA assay described to be sensitive, specific and reliable and could be adopted for serological investigation of small and large ruminants.