Detection of cervical intraepithelial neoplasia with HPVE6/E7 mRNA among women with atypical squamous cells of unknown significance. Issue 2 (26th February 2017)

• Record Type: Journal Article
• Authors: Li, Ya; Rong, Shouhua; Zhi, Yanfang; Qiu, Cui; Fan, Tingting; Li, Xiaofu
• Is Part Of: International journal of gynaecology and obstetrics. Volume 137:Issue 2(2017)
• Page Start: 145
• Page End: 149
• Publication Date: 2017-02-26
• Subjects: ASCUS -- CIN2+ -- E6/E7 mRNA -- HPV -- Management; Gynecology -- Periodicals; Obstetrics -- Periodicals; Electronic journals; 618
• DOI: 10.1002/ijgo.12113
• Languages: English
• ISSNs: 0020-7292

Abstract

Abstract: Objective: To compare E6/E7 mRNA and HPV DNA assays for evaluating women with atypical cells of undetermined significance (ASCUS). Methods: The present prospective study enrolled patients with ASCUS undergoing HPV testing at Third Affiliated Hospital of Zhengzhou University, China, between September 1, 2013, and January 31, 2016. Patients with positive HPV DNA test results underwent screening by E6/E7 mRNA assay, and the accuracy of HPV DNA and E6/E7 mRNA testing were compared, with histology used for definitive diagnoses. Results: In total, 591 patients with ASCUS underwent HPV DNA screening, with 455 and 136 having positive and negative results, respectively; 252 patients with positive results and 66 with negative results underwent biopsy and histology testing and were included in the study. The sensitivity of the E6/E7 mRNA assay was similar to that of HPV DNA testing (88.2%, 95% confidence interval [CI] 77.6–94.4 vs 90.7%, 95%CI 81.2–95.9; P= 0.636) for the detection of cervical intraepithelial neoplasia grade 2+, and the specificity was higher (36.4%, 95%CI 29.6–43.9 vs 24.3%, 95%CI 19.1–30.3; P= 0.006). The area under the receiver operating characteristic curve was greater for E6/E7 mRNA testing compared with HPV DNA testing (0.658 vs 0.588). Conclusion: The higher specificity of the E6/E7 mRNA assay means it could be a promising technique in the management of women with ASCUS. Abstract : HPV E6/E7 mRNA assay demonstrated higher specificity for detecting CIN2+ compared with HPV DNA testing in women with atypical squamous cells of unknown significance.