Development of a Modular Ratiometric Fluorescent Probe for the Detection of Extracellular Superoxide. Issue 20 (23rd March 2017)

• Record Type: Journal Article
• Authors: Andina, Diana; Brambilla, Davide; Munzinger, Noah; Frei, Jasmine; Zivko, Cristina; Leroux, Jean‐Christophe; Luciani, Paola
• Is Part Of: Chemistry. Volume 23:Issue 20(2017)
• Page Start: 4765
• Page End: 4769
• Publication Date: 2017-03-23
• Subjects: extracellular -- fluorescent probe -- peptide nucleic acid -- ratiometric -- reactive oxygen species; Chemistry -- Periodicals; 540
• DOI: 10.1002/chem.201700563
• Languages: English
• ISSNs: 0947-6539

Abstract

Abstract: Extracellular detection of endogeneous analytes (e.g., superoxide) can provide important insights into mechanisms of homeostasis and diseases, such as tumorigenesis. A ratiometric probe with a fluorescent reference and an analyte‐specific switch‐on dye was developed. Detection of ROS in the extracellular milieu was ensured by connecting the two fluorophores with a modular peptide‐nucleic‐acid‐based linker. The ROS‐sensing ability was assessed and validated in cell‐free assays and in cell culture. Abstract : A ratiometric probe with a fluorescent reference and an analyte‐specific switch‐on dye was developed. Detection of superoxide in the extracellular milieu was ensured by connecting the two fluorophores with a modular peptide‐nucleic‐acid‐based linker.