A key role of nuclear factor Y in the refeeding response of fatty acid synthase in adipocytes. Issue 7 (27th March 2017)

• Record Type: Journal Article
• Authors: Nishi‐Tatsumi, Makiko; Yahagi, Naoya; Takeuchi, Yoshinori; Toya, Naoki; Takarada, Ayako; Murayama, Yuki; Aita, Yuichi; Sawada, Yoshikazu; Piao, Xiaoying; Oya, Yukari; Shikama, Akito; Masuda, Yukari; Kubota, Midori; Izumida, Yoshihiko; Matsuzaka, Takashi; Nakagawa, Yoshimi; Sekiya, Motohiro; Iizuka, Yoko; Kawakami, Yasushi; Kadowaki, Takashi; Yamada, Nobuhiro; Shimano, Hitoshi
• Is Part Of: FEBS letters. Volume 591:Issue 7(2017)
• Page Start: 965
• Page End: 978
• Publication Date: 2017-03-27
• Subjects: adipose tissue -- fatty acid -- transcription; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1002/1873-3468.12620
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : Fatty acid synthase ( Fasn ) is a key component of energy metabolism that is dynamically induced by food intake. Although extensive studies have revealed a number of transcription factors involved in the fasting/refeeding transition of Fasn expression in hepatocytes, much less evidence is available for adipocytes. Using the in vivo Ad‐luc analytical system, we identified the inverted CCAAT element (ICE) around −100 nucleotides in the Fasn promoter as a critical cis‐element for the refeeding response in adipocytes. Electrophoretic mobility shift assays and chromatin immunoprecipitation show that nuclear factor Y (NF‐Y) binds to ICE specifically in refeeding states. Notably, the NF‐Y binding to ICE is differently regulated between adipocytes and hepatocytes. These findings provide insights into the specific mechanisms controlling energy metabolism in adipocytes. Abstract :