Determination of Staphylococcus aureus B-1266 by an Enzyme-Free Electrochemical Immunosensor Incorporating Magnetite Nanoparticles. (13th April 2017)

• Record Type: Journal Article
• Authors: Kozitsina, A.; Svalova, T.; Malysheva, N.; Glazyrina, Yu.; Matern, A.; Rusinov, V.
• Is Part Of: Analytical letters. Volume 50:Number 6(2017)
• Page Start: 924
• Page End: 935
• Publication Date: 2017-04-13
• Subjects: Bacteria detection -- biosensor -- cyclic voltammetry -- enzyme-free immunosensor; Chemistry, Analytic -- Periodicals; Chemistry, Analytic -- Abstracts; 543
• DOI: 10.1080/00032719.2016.1204312
• Languages: English
• ISSNs: 0003-2719

Abstract

ABSTRACT: A simple and inexpensive immunosensor is reported for the rapid determination of Staphylococcus aureus B-1266 that uses Fe3 O4 –SiO2 –NH2 nanoparticles as the direct signal label. The electrochemical immunoassay procedure includes the incubation of bacteria with excess magnetite nanoparticles, the magnetic separation of the free nanoparticles, a labeled immunocomplex formation on the surface of a planar electrode, and the electrochemical response from the magnetite nanoparticles in the immunocomplex. The electrochemical immunosensor allows for the selective and accurate detection of S. aureus from 10 to 105 CFU mL −1 with a relative standard deviation lower than 10%. The limit of detection was 8.7 CFU mL −1 .