Metabolic re-wiring of isogenic breast epithelial cell lines following epithelial to mesenchymal transition. (28th June 2017)

• Record Type: Journal Article
• Authors: Halldorsson, Skarphedinn; Rohatgi, Neha; Magnusdottir, Manuela; Choudhary, Kumari Sonal; Gudjonsson, Thorarinn; Knutsen, Erik; Barkovskaya, Anna; Hilmarsdottir, Bylgja; Perander, Maria; Mælandsmo, Gunhild M.; Gudmundsson, Steinn; Rolfsson, Óttar
• Is Part Of: Cancer letters. Volume 396(2017)
• Page Start: 117
• Page End: 129
• Publication Date: 2017-06-28
• Subjects: EMT -- Metabolism -- Genome scale models -- Breast cancer; EMT Epithelial to mesenchymal transition -- MET Mesenchymal to epithelial transition -- RNA-seq RNA sequencing -- UPLC-MS Ultra-performance liquid chromatography mass-spectrometry -- GEM Genome scale metabolic model -- FBA Flux balance analysis -- GPR Gene-Protein-Reaction association -- TF Transcription factor; Cancer -- Periodicals; Neoplasms -- Periodicals; Cancer -- Périodiques; Electronic journals; 616.994
• DOI: 10.1016/j.canlet.2017.03.019
• Languages: English
• ISSNs: 0304-3835

Abstract

Abstract: Epithelial to mesenchymal transition (EMT) has implications in tumor progression and metastasis. Metabolic alterations have been described in cancer development but studies focused on the metabolic re-wiring that takes place during EMT are still limited. We performed metabolomics profiling of a breast epithelial cell line and its EMT derived mesenchymal phenotype to create genome-scale metabolic models descriptive of both cell lines. Glycolysis and OXPHOS were higher in the epithelial phenotype while amino acid anaplerosis and fatty acid oxidation fueled the mesenchymal phenotype. Through comparative bioinformatics analysis, PPAR-γ1, PPAR- γ2 and AP-1 were found to be the most influential transcription factors associated with metabolic re-wiring. In silico gene essentiality analysis predicts that the LAT1 neutral amino acid transporter is essential for mesenchymal cell survival. Our results define metabolic traits that distinguish an EMT derived mesenchymal cell line from its epithelial progenitor and may have implications in cancer progression and metastasis. Furthermore, the tools presented here can aid in identifying critical metabolic nodes that may serve as therapeutic targets aiming to prevent EMT and inhibit metastatic dissemination.