A cross species study of heterogeneity in fear extinction learning in relation to FKBP5 variation and expression: Implications for the acute treatment of posttraumatic stress disorder. (April 2017)

• Record Type: Journal Article
• Authors: Galatzer-Levy, Isaac R.; Andero, Raül; Sawamura, Takehito; Jovanovic, Tanja; Papini, Santiago; Ressler, Kerry J.; Norrholm, Seth Davin
• Is Part Of: Neuropharmacology. Volume 116(2017)
• Page Start: 188
• Page End: 195
• Publication Date: 2017-04
• Subjects: Posttraumatic stress -- FKBP5 -- mRNA -- Threat extinction -- Fear extinction -- Dexamethasone -- Resilience -- Translational model -- Latent growth mixture modeling; Neuropsychopharmacology -- Periodicals; Autonomic Agents -- Periodicals; Neuropsychopharmacologie -- Périodiques; Neuropsychopharmacology; Periodicals; Electronic journals; 615.78
• DOI: 10.1016/j.neuropharm.2016.12.023
• Languages: English
• ISSNs: 0028-3908

Abstract

Abstract: Deficits in fear extinction learning are hypothesized to underlie the development of posttraumatic stress disorder (PTSD). Such deficits may, in part, be due to genetic and epigenetic variation in the stress related gene FKBP5 . Conversely, altering FKBP5 epigenetic responses during memory consolidation may rescue extinction deficits making it a target for acute intervention to prevent the development of PTSD. Study 1 (Humans) examines if FKBP5 single nucleotide polymorphisms (SNPs) and PTSD symptom domains (re-experiencing, avoidance/numbing, hyperarousal) are associated with abnormal fear extinction phenotypes identified using latent growth mixture modeling (LGMM). Study 2 (Mice) tests if increasing doses of dexamethasone administered prior to extinction alters Fkbp5 mRNA production in the amygdala after extinction and recall and prevents the development of abnormal extinction phenotypes. In humans, abnormal extinction was associated with the TT homozygous genotype of FKBP5 SNPs RS9470080 and RS1360780, and hyperarousal symptoms. In mice, dexamethasone 300 μg/kg was associated with increased amygdala Fkbp5 mRNA following extinction and robust extinction learning while lower doses were not associated with amygdala Fkbp5 mRNA or differences in extinction learning. Further, mice that extinguished on dexamethasone 300 μg/kg maintained low levels of freezing behavior during recall training while mRNA levels were no longer elevated. Together, findings indicate that FKBP5 confers risk for fear extinction deficits. However, this risk may be ameliorated by increasing fkbp5 mRNA expression in the amygdala during memory consolidation making this mechanism a plausible point of acute intervention to prevent the development of PTSD. Highlights: Distinct trajectories of extinction learning are present across specifies (humans & mice). Human trajectories are associated with variation in FKBP5 and hyper-arousal symptomatology of PTSD. Trajectories in mice are associated with fkbp5 mRNA expression in the amygdala. In mice, fkbp5 amygdala mRNA expression and extinction trajectory is altered by dexamethasone 300 μg/kg but not lower dosages.