Β1, 6‐GlcNAc Linkage to the Core Glycan on TIMP‐1 Affects Its Gelatinase Inhibitory Activities: Aberrantly Glycosylated TIMP‐1‐MMP2 Complex Modeling Shows Weaker Interaction Compared to Bi‐antennary Glycosylated TIMP‐1. (17th February 2017)

• Record Type: Journal Article
• Authors: Lee, Jin Kak; Han, Won Seok; Lee, Jun‐Seok; Kim, Yong‐Sam; Ko, Jeong‐Heon; Yoo, Jong Shin; Yoon, Chang No
• Is Part Of: Bulletin of the Korean Chemical Society. Volume 38:Number 3(2017)
• Page Start: 384
• Page End: 388
• Publication Date: 2017-02-17
• Subjects: Metalloproteinase‐1 -- Glycan structure -- Glycan interaction -- β1, 6‐N‐acetylglucosamine linkage; Chemistry -- Periodicals; 540.5
• DOI: 10.1002/bkcs.11096
• Languages: English
• ISSNs: 0253-2964

Abstract

Abstract : Matrix metalloproteinases (MMPs) are proteolytic enzymes that can regulate the tumor microenvironment. Metalloproteinase‐1 (TIMP‐1) is a MMP inhibitor that plays a critical role in the invasion and migration of cancer cells. N ‐acetylglucosaminyltransferase‐V (GnT‐V) catalyzes the attachment of a β1, 6‐ N ‐acetylglucosamine (GlcNAc) linkage to the core glycan, and TIMP‐1 has identified target proteins for GnT‐V. Recent research reveals that aberrantly glycosylated TIMP‐1 showed a weaker inhibition on gelatinase, and that this aberrancy of glycosylation was closely related with cancer cell invasion and metastasis. However, the mechanism of action of glycan modification is not known at the molecular level. In this study, the bi‐antennary and aberrant glycan structures were determined by mass spectrometry, and a model of the glycosylated TIMP‐1‐MMP2 complex was constructed to study the effects of glycosylation on the inhibitory activity of TIMP‐1. Then this model was used to examine the effects of attaching a β1, 6‐GlcNAc linkage to the core glycan on the interaction of TIMP‐1 with MMP2. The gelatinase inhibitory activity is decreased when additional a β1, 6‐GlcNAc moiety is linked to the core glycan on TIMP‐1. The modeled structure of the glycosylated TIMP‐1‐MMP2 complex reveals how aberrant N‐linked glycan hinders the interaction of these molecules. Abstract : Aberrantly glycosylated TIMP1‐MMP2 complex modeling shows weaker interaction compared to biantennary glycosylated TIMP1. The N53‐linked glycan mainly interacts with the collagenase‐like 1 region, and the N101‐linked glycan interacts with the fibronectin type II‐like (FIB) and hemopexin‐like (HPX) domain.