Structural elucidation and immunostimulating property of a novel polysaccharide extracted from an edible mushroom Lentinus fusipes. (10th February 2017)

• Record Type: Journal Article
• Authors: Manna, Dilip K.; Maity, Prasenjit; Nandi, Ashis K.; Pattanayak, Manabendra; Panda, Bibhash C.; Mandal, Amit K.; Tripathy, Satyajit; Acharya, Krishnendu; Sahoo, Atish K.; Gupta, Nibha; Roy, Somnath; Islam, Syed S.
• Is Part Of: Carbohydrate polymers. Volume 157(2017)
• Page Start: 1657
• Page End: 1665
• Publication Date: 2017-02-10
• Subjects: Lentinus fusipes -- Heteroglycan -- NMR studies -- Biological properties; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2016.11.048
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: A heteroglycan (PS-II) was isolated from the mushroom Lentinus fusipes. The PS-II consisted of glucose and galactose. Its structure was established by chemical and 1D/2D NMR studies. This molecule showed Biological activities. Abstract: A water soluble heteroglycan (PS-II) with an average molecular weight ∼ 60 kDa was isolated from the hot aqueous extract of an edible mushroom Lentinus fusipes . The structural characterization of PS-II was carried out using total acid hydrolysis, methylation analyses, periodate oxidation, Smith degradation and 1D/2D NMR experiments. Total acid hydrolysis indicated the presence of D-galactose and D-glucose in a molar ratio of approximately 1:1. The chemical and NMR analyses revealed that the proposed repeating unit of the PS-II had a backbone chain consisting of three (1 → 6)-linked α-d -galactopyranosyl residue and two (1 → 6)-linked β-d -glucopyranosyl residues, one of the β-d -glucopyranosyl residue was branched at O -3 position with a terminal β-d -glucopyranosyl. The PS-II exhibited significant in vitro splenocyte and macrophage activations with optimum dose of 20 μg/ml and 80 μg/ml respectively. Flow cytometry study revealed the protective role of the PS-II against nicotine stimulated lymphocytes. Moreover, the ROS scavenging property of PS-II was also established using DPPH radical scavenging assay.