Alginate- and gelatin-based bioactive photocross-linkable hybrid materials for bone tissue engineering. (10th February 2017)

• Record Type: Journal Article
• Authors: Lewandowska-Łańcucka, Joanna; Mystek, Katarzyna; Mignon, Arn; Van Vlierberghe, Sandra; Łatkiewicz, Anna; Nowakowska, Maria
• Is Part Of: Carbohydrate polymers. Volume 157(2017)
• Page Start: 1714
• Page End: 1722
• Publication Date: 2017-02-10
• Subjects: Alginate -- Gelatin -- Hydrogels -- Silica -- Tissue engineering; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2016.11.051
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: Hybrids from photo-crosslinked alginate/gelatin hydrogels and SiO2 were obtained. Mechanical properties of the resulting organic-inorganic hybrids might be adjusted. Addition of SiO2 does not compromise cytocompatibility of developed materials. Hybrids present good properties to be evaluated as a novel bioactive scaffolds. Abstract: The paper presents the synthesis, the physico-chemical and the biological properties of novel hybrid materials prepared from photo-crosslinked gelatin/alginate-based hydrogels and silica particles exhibiting potential for the regeneration of bone tissue. Both alginate and gelatin were functionalized with methacrylate and methacrylamide moieties, respectively to render them photo-crosslinkable. Submicron silica particles of two sizes were dispersed within three types of polymeric sols including alginate, gelatin, and gelatin/alginate blends, which were subsequently photo-crosslinked. The swelling ratio, the gel fraction and the mechanical properties of the hybrid materials developed were examined and compared to these determined for reference hydrogel matrices. The in vitro cell culture studies have shown that the prepared materials exhibited biocompatibility as they supported both MEFs and MG-63 mitochondrial activity. Finally, the in vitro experiments performed under simulated body fluid conditions have revealed that due to inclusion of silica particles into the biopolymeric hydrogel matrices the mineralization was successfully induced.