The use of heparin chemistry to improve dental osteogenesis associated with implants. (10th February 2017)

• Record Type: Journal Article
• Authors: Lee, Sang Jin; Bae, Min Soo; Lee, Deok Won; Heo, Dong Nyoung; Lee, Donghyun; Heo, Min; Hong, Seoung-Jin; Kim, Joohyoung; Kim, Wan Doo; Park, Su A; Kwon, Il Keun
• Is Part Of: Carbohydrate polymers. Volume 157(2017)
• Page Start: 1750
• Page End: 1758
• Publication Date: 2017-02-10
• Subjects: Dental titanium implant -- Heparin -- Growth/differentiation factor-5 -- Periodontal ligament stem cells -- Dental bone tissue regeneration; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2016.11.062
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: Titanium surface was developed to selectively graft the polymer using bioactive chemistry. The developed hybrid titanium disc showed outstanding in vitro osteo-integration activities. The surface modified titanium implant exhibited significant bone tissue regeneration in a rabbit model. Abstract: In this study, we designed a hybrid Ti by heparin modifying the Ti surface followed by Growth/differentiation factor-5 (GDF-5) loading. After that, products were characterized by physicochemical analysis. Quantitative analysis of functionalized groups was also confirmed. The release behavior of GDF-5 grafted samples was confirmed for up to 21 days. The surface modification process was found to be successful and to effectively immobilize GDF-5 and provide for its sustained release behavior. As an in vitro test, GDF-5 loaded Ti showed significantly enhanced osteogenic differentiation with increased calcium deposition under nontoxic conditions against periodontal ligament stem cells (PDLSc). Furthermore, an in vivo result showed that GDF-5 loaded Ti had a significant influence on new bone formation in a rabbit model. These results clearly confirmed that our strategy may suggest a useful paradigm by inducing osseo-integration as a means to remodeling and healing of bone defects for restorative procedures in dentistry.