Biocompatible testing and physical properties of curdlan-grafted poly(vinyl alcohol) scaffold for bone tissue engineering. (10th February 2017)

• Record Type: Journal Article
• Authors: Hsieh, Wen-Chuan; Hsu, Chia-Chi; Shiu, Li-Yen; Zeng, Yu-Jing
• Is Part Of: Carbohydrate polymers. Volume 157(2017)
• Page Start: 1341
• Page End: 1348
• Publication Date: 2017-02-10
• Subjects: Curdlan -- Scaffold -- Biodegradable polymer -- In vivo cultivation; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2016.11.010
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: Curdlan were grafted to PVA to form a porous scaffold. The water absorbency of scaffold is up to 280%. The strength of the scaffold strength reaches 8–16 × 10 −3 MPa. In vitro experiments revealed that cells proliferate and grow in the scaffold material. In vivo tests revealed favorable cell proliferation and growth in a 3D scaffold. Abstract: Curdlan was grafted to poly(vinyl alcohol) (PVA) to form a porous scaffold. The grafted PVA-curdlan 3D scaffold was then examined by Fourier transform infrared spectroscopy (FT-IR). Grafting increased the water absorbency of the scaffold by 280%. Scanning electron microscopic (SEM) observations of the material revealed that the 3D scaffold was highly porous when it was fabricated using a homogenizer at 300 rpm. Compression testing revealed that, increasing the amount of curdlan increased the strength of the 3D scaffold to 8–16 × 10 −3 MPa. Over 28 days, various enzymes degraded the 3D scaffold, causing a weight loss of up to 20–40%. In vivo tests revealed favorable cell proliferation and growth in a 3D scaffold.