Structural elucidation and immunostimulatory activity of polysaccharide isolated by subcritical water extraction from Cordyceps militaris. (10th February 2017)

• Record Type: Journal Article
• Authors: Luo, Xiaoping; Duan, Yuqing; Yang, Wenya; Zhang, Haihui; Li, Changzheng; Zhang, Jixian
• Is Part Of: Carbohydrate polymers. Volume 157(2017)
• Page Start: 794
• Page End: 802
• Publication Date: 2017-02-10
• Subjects: Cordyceps militaris -- Subcritical water extraction (SWE) -- Purification -- Characterization -- Immunostimulatory activity; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2016.10.066
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: CMP was obtained by subcritical water extraction. CMP-W1 and CMP-S1 were purified. Preliminary structural features of CMP-W1 and CMP-S1 were investigated. CMP-W1 and CMP-S1 displayed immunostimulatory activity in vitro . Abstract: Water-soluble polysaccharides were obtained from Cordyceps militaris ( C. militaris ) (CMP) by subcritical water extraction (SWE). Two polysaccharides fractions, CMP-W1 and CMP-S1, were isolated from CMP using DEAE-52 cellulose and Sephadex G-150 column chromatography. The structural characteristics of CMP-W1 and CMP-S1 were investigated. The results showed that the molecular weight of CMP-W1 and CMP-S1 are 3.66 × 105 Da and 4.60 × 105 Da, respectively, and both of them were heteropolysaccharides composed ofd -mannose, d -glucose, d -galactose with the molar ratios of 2.84:1:1.29 and 2.05:1:1.09, respectively. FT-IR spectra analysis suggested that CMP-W1 and CMP-S1 belonged to pyranose form sugar and protein free. For immunostimulatory activity assay in vitro, CMP-W1 and CMP-S1 significantly promoted lymphatic spleen cell proliferation of mice. Therefore, the polysaccharides obtained from C. militaris by SWE can be used as potential natural immunostimulant in functional foods or medicine.