Enhancement of membrane stability on magnetic responsive hydrogel microcapsules for potential on-demand cell separation. (10th February 2017)

• Record Type: Journal Article
• Authors: Wen, Huiyun; Gao, Ting; Fu, Zizhen; Liu, Xing; xu, Jiatong; He, Yishu; Xu, Ningxia; Jiao, Ping; Fan, An; Huang, Saipeng; Xue, Weiming
• Is Part Of: Carbohydrate polymers. Volume 157(2017)
• Page Start: 1451
• Page End: 1460
• Publication Date: 2017-02-10
• Subjects: Magnetic alginate/chitosan microcapsule -- Microencapsulation -- Membrane stability -- Magnetic cell separation; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2016.11.022
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: A facile design on magnetic response hydrogel microcapsules was proposed for achieving on-demand fed-batch cell separation. The suitable mechanical stable membrane of magnetic microcapsules was established. The membrane stability of MAC microcapsules were both size and membrane crosslinking time dependency. The prepared magnetic microcapsules have enough membrane stability in 286 h multiple fed-bath operations with no leakage of incorporated SPIONs and cells. Abstract: It is of high interest to obtain hydrogel membranes with optimum mechanical stability, which is a prerequisite to the successful fabrication of hydrogel microcapsules for cell separation. In this work, we developed magnetic responsive alginate/chitosan (MAC) hydrogel microcapsules by co-encapsulation of microbial cells and superparamagnetic iron oxide nanoparticles (SPIONs) reacting under a high voltage electrostatic field. We investigated the influence of the molecular weight of chitosan, microcapsules size, and membrane crosslinking time on the swelling behavior of microcapsules as an indicator of stability of the membranes. The results demonstrated that the suitable membrane stability conditions were obtained by a crosslinking of the microspheres with a chitosan presenting a molecular weight of 70 kDa for 15–30 min resulting in a membrane thickness of approximately 30 mm. Considering the need of maintaining the cells inside the microcapsules, fermentation at 37 °C and at neutral pH was favorable. Moreover, the MAC microcapsules sizing between 300 and 380 μm were suitable for immobilizing Bacillus licheniformis in a 286 h multiple fed-bath operation with no leakage of the SPIONs and cells. Overall, the results of this study provided strategies for the rational design of magnetic microcapsules exhibiting suitable mechanical stable membranes.