Lactoferrin-coated polysaccharide nanoparticles based on chitosan hydrochloride/hyaluronic acid/PEG for treating brain glioma. (10th February 2017)

• Record Type: Journal Article
• Authors: Xu, Yurui; Asghar, Sajid; Yang, Liu; Li, Hongying; Wang, Zhilin; Ping, Qineng; Xiao, Yanyu
• Is Part Of: Carbohydrate polymers. Volume 157(2017)
• Page Start: 419
• Page End: 428
• Publication Date: 2017-02-10
• Subjects: CSH chitosan hydrochloride -- HA hyaluronic acid -- Cur Curcuminoid -- CS chitosan -- PEG20000 poly (ethylene glycol) 20000 -- DMEM/HG Dulbecco's modified eagle's medium/high glucose -- DMEM/F12 Dulbecco's modified eagle's medium/nutrient mixture F-12 -- HPLC high performance liquid chromatography -- TEM transmission electron microscope -- BBB blood-brain barrier -- MA modified amount -- ME modification efficiency -- PDI polydispersity index -- EE encapsulation efficiency -- DL drug loading -- BCECs brain capillary endothelial cell -- C6 rat glioma cells -- UI uptake index -- RUI relative uptake index -- PSNPs polysaccharide nanoparticles -- Cur-PSNPs Cur-loaded PSNPs -- LC-PSNPs long circulating PSNPs -- Lf lactoferrin -- Tf transferrin -- LfR Lf receptor -- LRP low-density lipoprotein receptor-related protein -- Lf-PSNPs Lf modified PSNPs -- CNS central nervous system -- MW molecular weight -- MWCO molecular weight cut-off -- FBS fetal bovine serum -- MTT 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide -- DMSO dimethylsulfoxide -- BCA bicin choninic acid -- PBS phosphate buffered saline -- DiI 1, 1′-dioctadecyl-3, 3, 3′, 3′-tetramethylindocarbocyanine perchlorate -- DiO 3, 3′-dioctadecyloxacarbocyanine perchlorate -- DiR 1, 1′-dioctadecyl-3, 3, 3′, 3′-tetramethylindotricarbocyanine -- TEER transendothelial electrical resistance -- FRET fluorescence resonance energy transfer -- Di-PSNPs DiO and DiI double loaded PSNPs; Polysaccharide nanoparticles -- Chitosan hydrochloride -- Hyaluronic acid -- Lactoferrin -- Curcuminoid -- Glioma targeting; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2016.09.085
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: Lf modified PSNPs based on HA/CSH/PEG20000 were prepared for targeting brain glioma. Modification efficiency and modified amount of Lf on Lf-Cur-PSNPs were determined. The cellular uptakes of different PSNPs were investigated in BCECs and C6 cells. Ex vivo imaging studies of Lf-PSNPs showed more accumulation in the brain than PSNPs. Dual targeting for BBB and glioma cells could be achieved with Lf-PSNP. Abstract: Curcuminoid (Cur) loaded polysaccharide nanoformulations with blood-brain barrier (BBB) penetration and brain targeting properties, based on hyaluronic acid (HA) and chitosan hydrochloride (CSH) (Lf-Cur-PSNPs) were developed as a novel delivery system for malignant glioma. Formulations were investigated for physicochemical characteristics, cytotoxicity, uptake, and BBB penetration. The results showed that LfM -Cur-PSNPs (concentration of Lf was 0.5 mg/mL) were preferentially taken up by brain capillary endothelial cells than Cur-PSNPs at any time. After crossing the BBB, LfM -Cur-PSNPs remained largely intact and were more effective in targeting glioma cells (C6). In vivo imaging studies in mice exposed LfM -PSNPs could effectively permeate BBB and preferentially accumulate in the brain (2.39 times greater than PSNPs). Moreover, PSNPs were detected in brain up to 72 h. This data indicates that Lf-Cur-PSNPs could effectively target and accumulate within the gliomas after enhanced permeation through BBB, thus should be further explored for their potential in CNS maliganancies.