The effect of preparation processes on the physicochemical characteristics and antibacterial activity of chitooligosaccharides. (10th February 2017)

• Record Type: Journal Article
• Authors: Sánchez, Á.; Mengíbar, M.; Rivera-Rodríguez, G.; Moerchbacher, B.; Acosta, N.; Heras, A.
• Is Part Of: Carbohydrate polymers. Volume 157(2017)
• Page Start: 251
• Page End: 257
• Publication Date: 2017-02-10
• Subjects: Chitooligosaccharides -- Chitosanase -- Chemical depolymerization -- Escherichia coli -- Listeria monocytogenes; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2016.09.055
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: Chitosan oligosaccharides (COS) were prepared by an enzymatic one-step process (P1). COS P2 were prepared by two-step process combining chemical and enzymatic reaction. COS P2 was composed of a great proportion of fully deacetylated sequences (63%). COS P1 showed the strongest growth inhibition for E. coli and L. monocytogenes . N-acetyl-glucosamine sequences are necessary to exert good antibacterial capacity. Abstract: The bioactivities of chitooligosaccharides are markedly influenced by the degree of acetylation, degree of polymerization or molecular weight and pattern of acetylation. Thus, it is crucial to identify reproducible processes that will give rise to well-defined chitooligosaccharides and establish methods for their posterior physicochemical characterization in order to advance in the knowledge of their bioactivity. Chitooligosaccharides were prepared by two different processes. The first used chitosanase enzymatic hydrolysis and the second consisted of a two-step procedure based on chemical hydrolysis followed by chitosanase hydrolysis. Chitooligosaccharides produced in the second process were composed of 63 % of fully deacetylated sequences and inhibited the growth of Escherichia coli and Listeria monocytogenes . Better antibacterial activity was found for those obtained in the first process composed of 27 % of fully deacetylated sequences. Therefore, a low percentage of free amino groups and the presence of acetylated sequences are necessary in these molecules to exert good antibacterial capacity.