Site-specific antibody modification and immobilization on a microfluidic chip to promote the capture of circulating tumor cells and microemboli. Issue 29 (29th March 2017)

• Record Type: Journal Article
• Authors: Lai, Chian-Hui; Choon Lim, Syer; Wu, Liang-Chun; Wang, Chien-Fang; Tsai, Wen-Sy; Wu, Han-Chung; Chang, Ying-Chih
• Is Part Of: Chemical communications. Volume 53:Issue 29(2017)
• Page Start: 4152
• Page End: 4155
• Publication Date: 2017-03-29
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c7cc00247e
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Antibody coating via Fc-domain site-specific immobilization improves capture efficiency of circulating tumor cells and microemboli in microfluidics. Abstract : We design and synthesize EpCAM antibodies with Fc-domain site-specific linkers that allow preferential alignment when coated on microfluidic devices for capturing circulating tumor cells (CTCs) from colorectal cancer patients. The aligned coating is shown to increase the capture efficiency of CTCs and microemboli by 1.6 and 3.0-fold, respectively (both P < 0.05).