Colorimetric detection of HVA by self-assembly of Au nanorods with DNA double helices to give side-by-side and end-to-end structures. Issue 23 (1st March 2017)

• Record Type: Journal Article
• Authors: Chang, Lin; Khan, Younas; Li, Lidong; Yang, Nan; Yin, Penggang; Guo, Lin
• Is Part Of: RSC advances. Volume 7:Issue 23(2017)
• Page Start: 13896
• Page End: 13903
• Publication Date: 2017-03-01
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c6ra28408f
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : Simple, less time consuming and sensitive self-assembly and colorimetric detection of HVA. Abstract : In this article we present the self-assembly of gold nanorods with DNA double helices into side-by-side and end-to-end structures by the addition of HVA (hepatitis A virus Vall7 polyprotein gene). Between every two gold nanorods, there is a distance of 10.2 nm which agrees with the length of DNA. By observing the change in color with the naked eye or UV-Vis spectral absorbance, HVA concentration can be quantitatively measured. The colorimetric detection has a linear relationship between the UV-Vis spectral absorbance and the concentration, with a detectable range of 0.05 pM to 35 pM. The standard deviation " R 2 " value was found to be >0.99. This method is simple, time economic and highly sensitive, and a lower limit of detection up to 0.05 pM HVA can be achieved with great selectivity.