Heat shock 70 kDa protein cognate 5 involved in WSSV toleration of Litopenaeus vannamei. (July 2017)

• Record Type: Journal Article
• Authors: Yuan, Kai; Yuan, Feng-Hua; He, Hong-Hui; Bi, Hai-Tao; Weng, Shao-Ping; He, Jian-Guo; Chen, Yi-Hong
• Is Part Of: Developmental and comparative immunology. Volume 72(2017)
• Page Start: 9
• Page End: 20
• Publication Date: 2017-07
• Subjects: Litopenaeus vannamei -- ER-stress -- Unfolded protein response -- Heat shock protein cognate 5 -- White spot syndrome virus; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2017.02.003
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: The expression levels of 97 unigenes encoding heat shock proteins of Litopenaeus vannamei was scanned, and ten of them were significantly induced by white spot syndrome virus (WSSV). Among these genes, heat shock 70 kDa protein cognate 5 ( LvHSC70-5 ) was upregulated to the highest extent and subjected to further studies. Subcellular localization assay revealed that LvHSC70-5 was located in the mitochondria. Aside from WSSV infection, unfolded protein response activation and thermal stress could also upregulate LvHSC70-5 . Results of reporter gene assay demonstrated that promoter of LvHSC70-5 was activated by L . vannamei heat shock factor protein 1, activating transcription factor 4 and thermal stress. A decrease in the expression of LvHSC70-5 could reduce the aggregation of proteins in hemocytes and the cumulative mortality of WSSV-infected L . vannamei . LvHSC70-5 in L . vannamei hemocytes was upregulated by mild thermal stress. In addition, mild thermal stress, decreased the copy number of WSSV in shrimp muscle and the cumulative mortality of WSSV-infected L . vannamei . Therefore, collecting results suggested that LvHSC70-5 should be involved in WSSV toleration of shrimp L . vannamei . Highlights: LvHSC70-5 is upregulated by UPR activation and thermal stress in L. vannamei . LvHSC70-5 is activated by L . vannamei HSF1, ATF4 and thermal stress. LvHSC70-5 reduces the aggregation of proteins and the cumulative mortality of WSSV-infected shrimp. Mild thermal stresses decrease the copy number of WSSV and the cumulative mortality of WSSV-infected L . vannamei .