Redescription and molecular characterization of Anoplocephala manubriata, Railliet et al., 1914 (Cestoda: Anoplocephalidae) from a Sri Lankan wild elephant (Elephas maximus). Issue 3 (June 2017)

• Record Type: Journal Article
• Authors: Perera, K.U.E.; Wickramasinghe, Susiji; Perera, B.V.P.; Bandara, K.B.A.T.; Rajapakse, R.P.V.J.
• Is Part Of: Parasitology international. Volume 66:Issue 3(2017:Jun.)
• Page Start: 279
• Page End: 286
• Publication Date: 2017-06
• Subjects: Anoplocephala -- Taxonomy -- ITS-2 -- 28S -- COX1 -- Elephant; Parasitology -- Periodicals; Parasites -- Periodicals; Parasitic Diseases -- Periodicals; Parasitology -- Periodicals; Parasitologie -- Périodiques; 571.99905
• DOI: 10.1016/j.parint.2017.02.007
• Languages: English
• ISSNs: 1383-5769

Abstract

Abstract: The present work provides a detailed morphological and molecular description of Anoplocephala manubriata in elephants. Adult worms were recovered during an autopsy of a wild elephant in Elephant Transit Home, Udawalawe, Sri Lanka. Necropsy findings revealed a severe cestode infection in the small intestine. These tapeworms were tightly attached to the intestinal mucosae, resulted in hyperemic thickened intestinal mucosae, variable size irregular well-demarcated multifocal ulcerative regions sometimes covered with necrotic membranes and variable size, diffuse, well-demarcated raised nodular masses were evident in the small intestine. The article provides an account of the biology of A . manubriata and a comparative analysis of the morphology and morphometrics of Anoplocephala species that occur in different hosts. Phylogenetic analysis of the second internal transcribed spacer region (ITS-2), a portion of the 28S region and cytochrome oxidase subunit 1 (COX1) genes revealed that A . manubriata is closely associated with Anoplocephala species in horse in comparison to other Anoplocephalines. This study will enhance the current knowledge in taxonomy of elephant tapeworms and contribute to future phylogenetic studies. Graphical abstract: Gross image of adult worms of Anoplocephala manubriata and microscopic view and line drawing of a mature proglottid. Highlights: Detailed analysis of topological and morphometric features of Anoplocephala manubriata in Asian wild elephant. Comparative analysis of A . manubriata in the present study with the other species in the genus Anoplocephala . First molecular characterization of ITS-2 region, a portion of 28S region and COX1 gene of A . manubriata .