Cloning and expression of cry2Aa from native Bacillus thuringiensis strain SY49-1 and its insecticidal activity against Culex pipiens (Diptera: Culicidae). (April 2017)

• Record Type: Journal Article
• Authors: Yilmaz, Semih; Azizoglu, Ugur; Ayvaz, Abdurrahman; Temizgul, Ridvan; Atciyurt, Zehra Büşra; Karabörklü, Salih
• Is Part Of: Microbial pathogenesis. Volume 105(2017)
• Page Start: 81
• Page End: 85
• Publication Date: 2017-04
• Subjects: Bacillus thuringiensis -- Cloning -- Bt SY49-1 -- cry2Aa18 -- Larvicidal activity -- Culex pipiens; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2017.02.016
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Bacillus thuringiensis (Berliner) ( Bt ) is well known for having toxicity against pest insects because of their ability to form endospores and broad-range activity of their parasporal inclusions. In this study, a new member of cry2A gene from previously characterized native B. thuringiensis SY49-1 strain was cloned, expressed and used for its activity against Culex pipiens (Diptera: Culicidae) larvae. The sequence analysis of the cloned cry2A gene revealed that it encodes a polypeptide of 633 aa residues with 99% identity to Cry2Aa protein with expected molecular weight of 70.7 kDa. Bacillus thuringiensis delta-endotoxin nomenclature committee designed our sequence as Cry2Aa18 being a new member of Bt toxins. Bioassays against last instar larvae of C. pipiens indicated that Cry2Aa18 has considerable toxicity with LC50 of 630 μg ml −1 . In order to prevent the spread of infectious diseases mediated by C . pipiens, this newly characterized cry2Aa18 gene could constitute as an important biological control tool for controlling mosquito larvae living in freshwater systems and can be used as a good alternative for minimizing the use of chemicals. Graphical abstract: Highlights: cry2Aa18 gene from our strain was cloned and sequenced. Bt nomenclature committee designed our sequence as Cry2Aa18 being a new member of Bt toxins. Newly characterized cry2Aa18 gene could constitute as an important biological control tool.