Controllable Activation of Nanoscale Dynamics in a Disordered Protein Alters Binding Kinetics. Issue 7 (7th April 2017)

• Record Type: Journal Article
• Authors: Callaway, David J.E.; Matsui, Tsutomu; Weiss, Thomas; Stingaciu, Laura R.; Stanley, Christopher B.; Heller, William T.; Bu, Zimei
• Is Part Of: Journal of molecular biology. Volume 429:Issue 7(2017)
• Page Start: 987
• Page End: 998
• Publication Date: 2017-04-07
• Subjects: EBD Ezrin-binding domain -- FERM 4.1 protein, ezrin, radixin and moesin -- NSE neutron spin echo spectroscopy -- SPR surface plasmon resonance -- SEC size-exclusion chromatography -- SEC-SAXS SEC small angle X-ray scattering -- SANS small angle neutron scattering -- SSRL Stanford Synchrotron Radiation Light Source -- SNS Spallation Neutron Source; nanoscale protein motion -- disordered protein -- protein binding kinetics -- neutron spin echo spectroscopy -- protein dynamics; Molecular biology -- Periodicals; Biology -- Periodicals; Biochemistry -- Periodicals; Bacteriology -- Periodicals; Molecular Biology -- Periodicals; Biochemistry -- Periodicals; Biologie moléculaire -- Périodiques; Biologie -- Périodiques; Biochimie -- Périodiques; Moleculaire biologie; Biochemistry; Biology; Molecular biology; Periodicals; 572.805
• DOI: 10.1016/j.jmb.2017.03.003
• Languages: English
• ISSNs: 0022-2836

Abstract

Abstract: The phosphorylation of specific residues in a flexible disordered activation loop yields precise control of signal transduction. One paradigm is the phosphorylation of S339/S340 in the intrinsically disordered tail of the multi-domain scaffolding protein NHERF1, which affects the intracellular localization and trafficking of NHERF1 assembled signaling complexes. Using neutron spin echo spectroscopy (NSE), we show salt-concentration-dependent excitation of nanoscale motion at the tip of the C-terminal tail in the phosphomimic S339D/S340D mutant. The "tip of the whip" that is unleashed is near the S339/S340 phosphorylation site and flanks the hydrophobic Ezrin-binding motif. The kinetic association rate constant of the binding of the S339D/S340D mutant to the FERM domain of Ezrin is sensitive to buffer salt concentration, correlating with the excited nanoscale dynamics. The results suggest that electrostatics modulates the activation of nanoscale dynamics of an intrinsically disordered protein, controlling the binding kinetics of signaling partners. NSE can pinpoint the nanoscale dynamics changes in a highly specific manner. Graphical Abstract: Highlights: Nanoscale protein dynamics in an intrinsically disordered protein affects protein–protein interactions in a largely unknown fashion because it is experimentally difficult to probe protein dynamics on nanoscales. By employing the novel technique of NSE, we are able to reveal nanoscale dynamics changes in a disordered protein. Our theoretical analysis shows that NSE pinpoints the nanoscale dynamics changes in a highly specific manner. Debye–Hückel analysis indicates that changes in kinetic association rate constant at different ionic strengths cannot be explained by electrostatic effects alone. This study shows that nanoscale dynamics in a disordered protein significantly influences the binding kinetics of signaling partners.