AKT-targeted anti-inflammatory activity of the methanol extract of Chrysanthemum indicum var. albescens. (6th April 2017)

• Record Type: Journal Article
• Authors: Yang, Woo Seok; Kim, Donghyun; Yi, Young-Su; Kim, Ji Hye; Jeong, Hye Yoon; Hwang, Kyeonghwan; Kim, Jong-Hoon; Park, Junseong; Cho, Jae Youl
• Is Part Of: Journal of ethnopharmacology. Volume 201(2017)
• Page Start: 82
• Page End: 90
• Publication Date: 2017-04-06
• Subjects: Civ-ME Chrysanthemum indicum var. albescens methanol extract -- Ci-ME Chrysanthemum indicum methanol extract -- LPS lipopolysaccharide -- NO nitric oxide -- PG prostaglandin -- MTT 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide -- iNOS inducible nitric oxide synthase -- TNF-α tumor necrosis factor-alpha -- GAPDH glyceraldehyde 3-phosphate dehydrogenase -- NF-κB nuclear factor-kappa B -- AP-1 activator protein-1 -- RT-PCR reverse transcriptase-polymerase chain reaction -- MyD88 myeloid differentiation primary response gene (88) -- TRIF TIR-domain-containing adapter-inducing interferon-β -- PEI polyethylenimine -- IκBα inhibitor of kappa B alpha -- IKK IκB kinase; Chrysanthemum indicum var. albescens -- NF-κB -- AKT -- Anti-inflammatory activity -- Macrophage; Ethnopharmacology -- Periodicals; Pharmacognosy -- Periodicals; Herbs -- Periodicals; Herbs -- Periodicals; Pharmacognosy -- Periodicals; Pharmacognosie -- Périodiques; Herbes -- Périodiques; 615.1
• DOI: 10.1016/j.jep.2017.03.001
• Languages: English
• ISSNs: 0378-8741

Abstract

Abstract: Ethnopharmacological relevance: Wild chrysanthemum ( Chrysanthemum indicum ) is one of well-known medicinal plants traditionally used in Korea and China. As a variant of wild chrysanthemum, white wild chrysanthemum ( Chrysanthemum indicum var. albescens) is also ethnopharmacologically applied to treat various symptoms such as inflammatory diseases. Aim of study: Although the anti-inflammatory activity of Chrysanthemum indicum has been reported, the anti-inflammatory activity and underlying molecular mechanism of white wild chrysanthemum are poorly understood. Materials and methods: The effects of Chrysanthemum indicum var. albescens methanol extract (Civ-ME) on the production of inflammatory mediators, expression of pro-inflammatory genes, cell viability, and the activities of intracellular signaling molecules and transcription factors were investigated in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. Results: Civ-ME suppressed the production of both nitric oxide (NO) and prostaglandin E2 (PGE2 ) without cytotoxicity in LPS-stimulated RAW264.7 cells. Civ-ME was found to reduce the mRNA levels of inflammatory genes such as inducible NO synthase (iNOS) and tumor necrosis factor (TNF)-α and reduced NF-κB-mediated transcriptional activation. Civ-ME inhibited the nuclear translocation of NF-κB (p65 and p50), and its upstream signaling composed of IκBα and IKKα/β. An NF-κB luciferase reporter gene assay and an in vitro kinase assay confirmed that AKT1 and AKT2 might be direct pharmacological targets of Civ-ME. In addition, luteolin was identified by HPLC analysis as the main active pharmacological components of Civ-ME. Conclusion: Civ-ME exerts an anti-inflammatory effect by targeting AKT1 and AKT2 in the NF-κB signaling pathway in macrophage-mediated inflammatory responses. Graphical abstract: