Sodium chlorate induces DNA damage and DNA-protein cross-linking in rat intestine: A dose dependent study. (June 2017)

• Record Type: Journal Article
• Authors: Ali, Shaikh Nisar; Ansari, Fariheen Aisha; Arif, Hussain; Mahmood, Riaz
• Is Part Of: Chemosphere. Volume 177(2017)
• Page Start: 311
• Page End: 316
• Publication Date: 2017-06
• Subjects: NaClO3 -- Comet assay -- DNA damage -- DNA-protein cross-links -- Genotoxicity; DPC DNA-protein crosslinks -- EDTA ethylenediaminetetraacetic acid -- NaClO3 sodium chlorate -- ROS reactive oxygen species -- RPMI Roswell Park Memorial Institute 1640 -- PBS phosphate buffer saline -- SDS sodium dodecyl sulfate; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2017.03.018
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Sodium chlorate (NaClO3 ) is widely used in paper and pulp industries and as a non-selective herbicide. It is also a major by-product generated upon disinfection of drinking water by chlorine dioxide. In this study, we have investigated the genotoxicity of NaClO3 on the small intestine of rats. Adult male rats were divided into 5 groups: one control and four NaClO3 treated groups. The NaClO3 treated groups were given a single acute oral dose of NaClO3 (100, 250, 500 and 750 mg/kg body weight) and sacrificed 24 h later. Administration of NaClO3 caused significant DNA damage in a dose dependent manner in the rat intestine. This was evident from the comet assay which showed DNA strand breaks and was further confirmed by agarose gel electrophoresis and release of free nucleotides. Increased DNA protein cross-linking in NaClO3 administered groups showed formation of a critical lesion which hampers activities of proteins/enzymes involved in DNA repair, transcription and replication. Thus, oral administration of NaClO3 induces DNA damage in the rat intestine, probably through chlorate induced production of reactive oxygen species. Highlights: Oral administration of NaClO3 induces DNA damage in rat intestine. NaClO3 induces DNA-protein cross-link formation and DNA strand breaks. DNA fragmentation was observed from electrophoresis and colorimetric assays.