Role of vitamin E and D3 supplementation in Intra-Cytoplasmic Sperm Injection outcomes of women with polycystic ovarian syndrome: A double blinded randomized placebo-controlled trial. (April 2017)

• Record Type: Journal Article
• Authors: Fatemi, Farnaz; Mohammadzadeh, Afsaneh; Sadeghi, Mohammad Reza; Akhondi, Mohammad Mehdi; Mohammadmoradi, Shayan; Kamali, Koorosh; Lackpour, Nicknam; Jouhari, Sheida; Zafadoust, Simin; Mokhtar, Sara; Giahi, Ladan
• Is Part Of: Clinical nutrition ESPEN. Volume 18(2017)
• Page Start: 23
• Page End: 30
• Publication Date: 2017-04
• Subjects: Vitamin E -- Vitamin D3 -- Polycystic ovarian syndrome -- Female infertility -- Oxidative stress -- ICSI; PCOS Polycystic ovarian syndrome -- TAC Total Antioxidant Capacity -- MDA Malondialdehyde -- ICSI Intra-Cytoplasmic Sperm Injection -- ART Assisted Reproductive Technology -- OHSS Ovarian Hyper Stimulation Syndrome; Nutritionally induced diseases -- Periodicals; Metabolism -- Disorders -- Periodicals; 616.39005
• DOI: 10.1016/j.clnesp.2017.01.002
• Languages: English
• ISSNs: 2405-4577

Abstract

Summary: Background & aims: Ovulation induction has been proven to impose oxidative stress during ICSI treatment cycle. Also, data indicates that PCOS women show higher oxidative markers. Available data are not convincing about which antioxidant supplements have the potential to overcome oxidative stress in PCOS subjects. The aim of this trial was to investigate the possible role of combined vitamin E and D supplementation in the ICSI outcomes (oocyte number and quality, embryo number and quality, pregnancy rate) of PCOS subjects. Methods: A total of 105 PCOS infertile women scheduled for ICSI were enrolled in a double-blinded RCT to treatment group (vitamin E, 400 mg/day – and vitamin D3, 50, 000 IU/one in two weeks, n = 52) or placebo group (n = 53) for 8 weeks. The primary outcomes were implantation rate, pregnancy and clinical pregnancy rate. Secondary outcomes included oocyte quality, embryo quality, fertilization rate, alteration in serum MDA, TAC and vitamin D3 after treatment. Further, association between serum and follicular fluid Malondialdehyde (MDA), Total Antioxidant Capacity (TAC), and serum vitamin D3 level were assessed. Results: Pregnancy, clinical pregnancy and implantation rate were significantly higher in treatment group (P < 0.001). Data analysis in both groups revealed a significant increase in serum MDA compared to baseline and a significant decrease in serum TAC compared to baseline after treatment. Further analysis showed that there is a positive weak association between vitamin D level, implantation rate (P = 0.015) and increased clinical pregnancy (P = 0.037). No significant association was detected between either follicular fluid or serum MDA and TAC and ICSI outcomes. Conclusions: In conclusion, the findings of this trial do not add clinical support to the evidence that vitamins E and D3 may play a role in the success rate of IVF via an antioxidant mechanism. Registry code: IRCT2014081018662N1.