Effects of 17β-estradiol and 17α-ethinylestradiol on the embryonic development of the clearhead icefish (Protosalanx hyalocranius). (June 2017)

• Record Type: Journal Article
• Authors: Hu, Shuangqing; Zhang, Hongchang; Shen, Genxiang; Yuan, Zhejun; Xu, Ting; Ji, Rong
• Is Part Of: Chemosphere. Volume 176(2017)
• Page Start: 18
• Page End: 24
• Publication Date: 2017-06
• Subjects: Estrogen -- 17β-Estradiol -- 17α-Ethinylestradiol -- Protosalanx hyalocranius -- Fish embryo toxicity test; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2017.02.094
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Estrogenic effects of 17β-estradiol (E2) and 17α-ethinylestradiol (EE2) on animals have been widely reported. The high sensitivity of fish in the early-life stages to xenobiotics can be exploited to evaluate the developmental effects of environmentally relevant levels of E2 and EE2. In this work, clearhead icefish ( Protosalanx hyalocranius ) embryos (blastula stage) were exposed to E2 or EE2 at concentrations between 0.05 ng/L and 1 mg/L. The toxicity endpoints of mortality, teratogenesis, and hatching retardation were evaluated. The results showed that continuous exposure of the fish embryos/larvae to higher concentrations of E2 and EE2 dramatically increased mortality after 17 days, when the hatching period started. An E2 concentration of 8 μg/L (day 16) and an EE2 concentration of at 0.2 mg/L (day 18) induced maximum teratogenesis rates of 30% and 35%, respectively. Embryos exposed to 0.2 mg E2 or EE2/L had a significantly retarded hatching time compared to the control. Thus, although environmentally relevant concentrations E2 and EE2 are not lethal for P. hyalocranius embryos or larvae, their ability to induce teratogenesis and hatching retardation merits concern. Highlights: Big salangid, an important economic fish species in Yangtze River of China, was selected as test animal. E2 and EE2 disrupted teratogenesis and development delay of big salangid when nearing hatching. E2 has more significant effect on embryo than EE2.