Optogenetic Control of Ca2 + and Voltage-Dependent Large Conductance (BK) Potassium Channels. Issue 6 (24th March 2017)

• Record Type: Journal Article
• Authors: Mager, Thomas; Wood, Phillip G.; Bamberg, Ernst
• Is Part Of: Journal of molecular biology. Volume 429:Issue 6(2017)
• Page Start: 911
• Page End: 921
• Publication Date: 2017-03-24
• Subjects: ChR channelrhodopsin -- CatCh Calcium translocating Channelrhodopsin -- BK Big Potassium -- HEK human embryonic kidney -- BAPTA 1, 2-bis(o-aminophenoxy)ethane-N, N, N′, N′-tetraacetic acid -- Cav voltage-gated calcium; optogenetics -- BK channel -- channelrhodopsin -- calcium jump -- minimal invasive; Molecular biology -- Periodicals; Biology -- Periodicals; Biochemistry -- Periodicals; Bacteriology -- Periodicals; Molecular Biology -- Periodicals; Biochemistry -- Periodicals; Biologie moléculaire -- Périodiques; Biologie -- Périodiques; Biochimie -- Périodiques; Moleculaire biologie; Biochemistry; Biology; Molecular biology; Periodicals; 572.805
• DOI: 10.1016/j.jmb.2017.02.004
• Languages: English
• ISSNs: 0022-2836

Abstract

Abstract: Ca 2 + concentration jumps for the activation of Ca 2 + -dependent ion channels or transporters can be obtained either by fast solution exchange or by the use of caged Ca 2 + . Here, we report on an alternate optogenetic method for the activation of Ca 2 + and voltage-dependent large conductance (BK) potassium channels. This was achieved through the use of the light-gated channelrhodopsin 2 variant, CatCh(Calcium translocating Channelrhodopsin) with enhanced Ca, which produces locally [Ca 2 + ] in the μM range on the inner side of the membrane, without significant [Ca 2 + ] increase in the cytosol. BK channel subunits α and β1 were expressed together with CatCh in HEK293 cells, and voltage and Ca 2 + dependence were analyzed. Light activation of endogenous BK channels under native conditions in astrocytes and glioma cells was also investigated. Additionally, BK channels were used as sensors for the calibration of the [Ca 2 + ] on the inner surface of the cell membrane. Graphical Abstract: Highlights: Optogenetic approach for channel activation CatCh-mediated activation of BK potassium channel Light-controlled changes of submembrane calcium concentration Under hyperpolarization, the BK channel is only controlled by [Ca 2+ ]. Calcium concentration jumps in a minimal invasive and reversible manner.