Studies on expression pattern of toll-like receptor 5 (TLR5) in Edwardsiella tarda infected Pangasianodon hypophthalmus. Issue 63 (April 2017)

• Record Type: Journal Article
• Authors: Jayaramu, Poojashree Kachigere; Tripathi, Gayatri; Pavan Kumar, A.; Keezhedath, Jeena; Pathan, Mujahid Khan; Kurcheti, Pani Prasad
• Is Part Of: Fish & shellfish immunology. Issue 63(2017)
• Page Start: 68
• Page End: 73
• Publication Date: 2017-04
• Subjects: Toll like receptor -- Innate immunity -- E. tarda -- mRNA expression -- Real time quantitative PCR; LRR Leucine Rich Repeats -- MYD88 Myeloid Differentiation Primary Response 88 -- PRR Pattern Recognization Receptor -- PAMP Pathogen Associated Molecular Patterns -- NF-kB nuclear factor kappa B -- IPTG Isopropyl-β-d-thiogalactopyranoside -- M-MuLV-RT Moloney Murine Leukemia Virus reverse transcriptase -- X-gal/BCIG 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside -- PBS Phosphate Buffered Saline -- qPCR quantitative real time polymerase chain reaction -- ANOVA analysis of variance -- TAE Tris base-Acetic acid-EDTA -- BHI Brain Heart infusion -- SS agar Salmonella Shigella agar -- SPSS Statistical Package for Social Science; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2017.01.041
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: TLR5 is one of the important PRR (pathogen recognition receptors) and plays a fundamental role in pathogen recognition and activation of innate immune responses. It recognizes bacterial flagellin and stimulates the production of proinflammatory cytokines, through signalling via the adaptor protein MyD88. In this study, we characterized partial TLR5 (soluble form) gene from Pangasianodon hypophthalmus and analysed its expression profile upon challenge by Edwardsiella tarda. Bioinformatic analysis of gene sequence revealed a putative protein of 266 amino acids with four Leucine rich repeats. Quantitative expression analysis of TLR 5S showed its wide distribution in various organs and tissues. However, significant expression of TLR5S was observed in liver and spleen at 12 h (∼207.8 fold, p < 0.05). Significant upregulation was observed in kidney at 72 h.p.i. (50 folds, p < 0.05) indicating that the kidney provides longer protection almost till the activation of the adaptive immune system. This study enriches the knowledge of TLR5S in boosting the innate immunity against bacterial invasion in fish. Highlights: We identified and partially characterized the Toll like receptor 5 in P. hypophthalmus . The catfish TLR 5 gene is variously expressed in different immune organs at different time intervals against E. tarda infection. We studied histopathological changes in various tissues of catfish against E. tarda infection.