Death associated protein 1 (DAP 1) positively regulates virus replication and apoptosis of hemocytes in shrimp Marsupenaeus japonicus. Issue 63 (April 2017)

• Record Type: Journal Article
• Authors: Xia, Wen-Li; Kang, Li-Hua; Liu, Chang-Bin; Kang, Cui-Jie
• Is Part Of: Fish & shellfish immunology. Issue 63(2017)
• Page Start: 304
• Page End: 313
• Publication Date: 2017-04
• Subjects: Apoptosis -- Death-associated protein 1 (DAP1) -- Shrimp -- Anti-virus immunity -- White spot syndrome virus (WSSV); Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2017.02.014
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Death-associated protein 1 (DAP1) is a small proline-rich cytoplasmic protein that functions both in the apoptosis and autophage process of mammalian and in the clinical cancer of human. However, little knowledge is known about the homologue gene of DAP1 and its roles in the physiological process of invertebrates. In this paper, we report a novel function of DAP1 in the antivirus immunity of shrimp. A homologue gene of DAP1 was cloned from Marsupenaeus japonicus and named as Mjdap -1. The full-length of Mjdap -1 was 1761 bp with a 309 bp open reading frame that encoded 102 amino acids. Reverse transcription-PCR results showed that Mjdap- 1 was expressed in all tested tissues, including hemocytes, gills, intestines, stomach, heart, hepatopancreas, testes, and ovaries. In shrimp, Mjdap- 1 transcripts were up-regulated by white spot syndrome virus (WSSV) infection; Mjdap- 1 knockdown decreased the virus copy in vivo and the mortality of M. japonicus to WSSV challenge. Conversely, injecting the purified recombinant Mj DAP1 protein promoted the amplification of virus in shrimp. Flow cytometric assay showed, the virus infection-induced apoptosis of hemocytes was enhanced by Mj DAP1 protein injection and inhibited in Mj DAP1 knockdown shrimp. Furthermore, the expression of apoptosis-inducing factor (AIF) was regulated by Mjdap- 1, but the caspase transcripts were not affected. Our results suggested that Mj DAP1 facilitated the amplification of virus in shrimp, which may be attributed to the promotion of hemocyte apoptosis in an AIF-dependent manner. These results provided a new insight into the function of this protein that may be used for virus disease control. Highlights: DAP1 ( Mjdap -1) is identified in kuruma shrimp Marsupenaeus japonicus. Mjdap -1 is upregulated in several tissues after white spot syndrome virus challenge. Mjdap -1 facilitates the amplification of virus in shrimp. Mjdap -1 promotes the apoptosis of hemocytes in shrimp by affecting the expression of apoptosis inducing factor (AIF)gene.