Development of an oral mucosa equivalent using a porcine dermal matrix. Issue 3 (April 2017)

• Record Type: Journal Article
• Authors: Basso, F.G.; Hebling, J.; Marcelo, C.L.; de Souza Costa, C.A.; Feinberg, S.E.
• Is Part Of: British journal of oral and maxillofacial surgery. Volume 55:Issue 3(2017)
• Page Start: 308
• Page End: 311
• Publication Date: 2017-04
• Subjects: Oral keratinocytes -- organotypic cell culture -- oral mucosa; Mouth -- Surgery -- Periodicals; Maxilla -- Surgery -- Periodicals; Face -- Surgery -- Periodicals; Surgery, Plastic -- Periodicals; Dentistry, Operative -- Periodicals; Face -- surgery -- Periodicals; Mouth -- surgery -- Periodicals; Maxilla -- surgery -- Periodicals; Surgery, Oral -- Periodicals; Oral Surgical Procedures -- Periodicals; Dentistry, Operative -- Periodicals; Bouche -- Chirurgie -- Périodiques; Maxillaire supérieur -- Chirurgie -- Périodiques; Face -- Chirurgie -- Périodiques; Chirurgie dentaire -- Périodiques; Dentistry, Operative; Face -- Surgery; Maxilla -- Surgery; Mouth -- Surgery; Surgery, Plastic; Electronic journals; Periodicals; 617.52059
• DOI: 10.1016/j.bjoms.2016.09.019
• Languages: English
• ISSNs: 0266-4356

Abstract

Abstract: We evaluated the suitability of a porcine acellular dermal matrix for the development of a 3-dimensional oral mucosal equivalent using an ex vivo-produced oral mucosal equivalent (EVPOME). Oral keratinocytes were seeded in a submerged model, and then in an air-liquid interphase model, using Transwell ® inserts. EVPOME showed good cell viability and increased glucose consumption over time. Histological evaluation showed that stratified differentiated epithelium had formed in all matrices.