Aerobic and heterotrophic nitrogen removal by Enterobacter cloacae CF-S27 with efficient utilization of hydroxylamine. (May 2017)

• Record Type: Journal Article
• Authors: Padhi, Soumesh Kumar; Tripathy, Swetaleena; Mohanty, Sriprakash; Maiti, Nikhil Kumar
• Is Part Of: Bioresource technology. Volume 232(2017)
• Page Start: 285
• Page End: 296
• Publication Date: 2017-05
• Subjects: Heterotrophic nitrification -- Aerobic denitrification -- Hydroxylamine reductase -- Enterobacter cloacae -- qRT-PCR; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2017.02.049
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Hydroxylamine reductase ( hao ) involved in both nitrification and denitrification. 200 mg of external hydroxylamine utilized within 3 h of bacterial incubation. Degenerated PCR primer for hao gene of Enterobacteriaceae and Bacillaceae family. Enterobacter cloacae CF-S27 has tremendous potential to produce bacterial floc. Domestic wastewater used in fish culture system by the help of bacterial floc. Abstract: Heterotrophic bacterium, Enterobacter cloacae CF-S27 exhibited simultaneous nitrification and aerobic denitrification in presence of high concentration of hydroxylamine. With the initial nitrogen concentration of 100 mg L −1 h −1, ammonium, nitrate and nitrite removal efficiencies were 81%, 99.9% and 92.8%, while the corresponding maximum removal rates reached as high as 11.6, 15.1 and 11.2 mg L −1 h −1 respectively. Quantitative amplification by real time PCR and enzyme assay demonstrated that hydroxylamine reductase gene ( hao ) is actively involved in hetrotrophic nitrification and aerobic denitrification process of Enterobacter cloacae CF-S27. PCR primers were designed targeting amplification of hao gene from diversified environmental soil DNA. The strain Enterobacter cloacae CF-S27 significantly maintained the undetectable amount of dissolved nitrogen throughout 60 days of zero water exchange fish culture experiment in domestic wastewater.