Effects of chitosan-coated lipid-core nanocapsules on bovine sperm cells. (April 2017)

• Record Type: Journal Article
• Authors: de Castro Jorge Silva, Arthur; Remirão, Mariana Härter; Lucas, Caroline Gomes; Domingues, William Borges; Silveira, Tony; Paschoal, Julia Dame; Jornada, Denise S.; Corcine, Carine Dahl; Junior, Antonio Sergio Varela; Prado, Willian A.; Campos, Vinicius Farias; Seixas, Fabiana Kömmling; Guterres, Silvia S.; Pohlmann, Adriana R.; Collares, Tiago
• Is Part Of: Toxicology in vitro. Volume 40(2017)
• Page Start: 214
• Page End: 222
• Publication Date: 2017-04
• Subjects: Nanosystem -- Nanocapsules -- Chitosan -- Sperm -- Spermtoxicology; Toxicity testing -- In vitro -- Periodicals; Toxicology -- Periodicals; 615.9
• DOI: 10.1016/j.tiv.2017.01.017
• Languages: English
• ISSNs: 0887-2333

Abstract

Abstract: Toxicology studies have a pivotal role for selection of new nanosystems. As lipid-core nanocapsules (LNC) rise as a potential system not only for drug delivery but also for immunotherapy and gene therapy, the demand for models of toxic screening increases, and sperm arises as a promising model due to the easiness to evaluate its viability parameters. LNCs were coated with chitosan, chitosan-coated lipid-core nanocapsules (LNC-CS), in order to modify the nanocapsule surface. We evaluated the toxicity of LNC and LNC-CS after incubation with bovine sperm in different concentrations (2.5%, 5%, 10%, 20%, 40% and 80%) (v/v) and periods of exposure (0 h and 1 h). CASA parameters and flow cytometry assays were performed to assess LNC and LNC-CS effects. The results corroborated with previous studies indicating that there is no toxicity from LNCs and LNC-CS below 40% (v/v) concentration. Highlights: Lipid-core nanocapsules (LNC) were coated with chitosan (CS). Toxicity of LNC and LNC-CS after incubation with bovine sperm in different concentrations CASA parameters and flow cytometry assays were performed to assess LNC and LNC-CS effects. There is no toxicity from LNCs and LNC-CS above 40% (v/v) concentration.